Protein Info for BWI76_RS04250 in Klebsiella michiganensis M5al

Annotation: homoserine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00191: homoserine kinase
  • amino acids 2 to 308 (307 residues), 398.1 bits, E=9.7e-124
• PF00288: GHMP_kinases_N
  • amino acids 84 to 150 (67 residues), 60.3 bits, E=1.9e-20
• PF08544: GHMP_kinases_C
  • amino acids 211 to 283 (73 residues), 43 bits, E=4.9e-15

Best Hits

Swiss-Prot: 94% identical to KHSE_KLEP7: Homoserine kinase (thrB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00872, homoserine kinase [EC: 2.7.1.39] (inferred from 94% identity to kpe:KPK_4754)

MetaCyc: 87% identical to homoserine kinase (Escherichia coli K-12 substr. MG1655)
Homoserine kinase. [EC: 2.7.1.39]; 2.7.1.- [EC: 2.7.1.39]

Predicted SEED Role

"Homoserine kinase (EC 2.7.1.39)" in subsystem Methionine Biosynthesis or Threonine and Homoserine Biosynthesis (EC 2.7.1.39)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-threonine biosynthesis (2/2 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.39

Sequence Analysis Tools

See A0A285AY93 at UniProt or InterPro

Protein Sequence (309 amino acids)

>BWI76_RS04250 homoserine kinase (Klebsiella michiganensis M5al)
MVKVYAPASSANMSVGFDVLGAAVTPVDGTLLGDNVSVEAAASFSLQNVGRFASKLPTAP
QENIVYQCWERFCQEIGKKVPVAMTLEKNMPIGSGLGSSACSVVAALVAMNEFCGKPLNE
SRMLALMGEMEGRISGSIHYDNVAPCYLGGIQLMLEENGIISQQIPSFDEWLWVLAYPGI
KVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQPQLAAKLMKDVIAEPYRTKLLP
GFSEARQAAMEIGAQACGISGSGPTLFALCDKPETAQRVADWLATHYLQNQEGFVHICRL
DTAGARVVG