Protein Info for BWI76_RS04235 in Klebsiella michiganensis M5al

Annotation: tRNA/rRNA methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 signal peptide" amino acids 1 to 14 (14 residues), see Phobius details TIGR00050: RNA methyltransferase, TrmH family, group 1" amino acids 2 to 224 (223 residues), 229 bits, see alignment E=3.7e-72 PF00588: SpoU_methylase" amino acids 3 to 153 (151 residues), 92.9 bits, see alignment E=1e-30

Best Hits

Swiss-Prot: 76% identical to LAST_ECOLI: Uncharacterized tRNA/rRNA methyltransferase LasT (lasT) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 84% identity to eae:EAE_10680)

Predicted SEED Role

"tRNA:Cm32/Um32 methyltransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYC5 at UniProt or InterPro

Protein Sequence (228 amino acids)

>BWI76_RS04235 tRNA/rRNA methyltransferase (Klebsiella michiganensis M5al)
MRLSIVLVSPARAENVGAAARAMKTMGFSDMRIVDSDAHLQPAARWVAHGSGEILDNVKT
YATLEQALHDVDFTVATTARSRARFHYYATPQQLLPLLEEKAQWMHHAALVFGREDSGLT
NEELALADVLSGVQMVADYPSLNLGQSVMVYCYQLTSLLQNTPSTSASVDDNQLQALRLR
TRDLLERLGVEDDIKLTDWLQQRMGLLQQRDTAMLHRLLHDIEKNLPD