Protein Info for BWI76_RS04220 in Klebsiella michiganensis M5al

Annotation: two-component sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 184 to 206 (23 residues), see Phobius details PF00512: HisKA" amino acids 260 to 319 (60 residues), 53.5 bits, see alignment E=2e-18 PF02518: HATPase_c" amino acids 363 to 470 (108 residues), 82.8 bits, see alignment E=2.4e-27

Best Hits

Swiss-Prot: 76% identical to CREC_ECOLI: Sensor protein CreC (creC) from Escherichia coli (strain K12)

KEGG orthology group: K07641, two-component system, OmpR family, sensor histidine kinase CreC [EC: 2.7.13.3] (inferred from 84% identity to kva:Kvar_4397)

MetaCyc: 76% identical to sensory histidine kinase CreC (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Two-component response regulator CreC"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AYE6 at UniProt or InterPro

Protein Sequence (474 amino acids)

>BWI76_RS04220 two-component sensor histidine kinase (Klebsiella michiganensis M5al)
MRIGMRLLMGYFLIVAIAAWFVLSIFVQEIKPGVRRATEGTLIDTATLLAELAREDLLSA
DPQHGRLAQAFQTLHRQPINANIAGIKKVRNEYHVYMTDAQGKVVFDSAGNAQGQDYSRW
NDVWLTLRGQYGARSTRSDPNDDNSSVMYIAAPVTDNGRIIGVLSVGKPNLAMAPVIKRS
ERRILWAGGALLGIALLIGVAMVWWINFSIGKLVRYADSVTADRPLPLPDAGSSELRKLA
QALESMRLKLEGKNAIENYVYALTHELKSPLAAIRGAAEILREAPPPEVAARFTRNILAQ
NARMQALVENLLQQARLENRLEIAQRPVSVAALFRRLAEERDIALAAKNLTLRWQETSLT
VEGDGELLAQALGNLLDNAIDFTPAEGEIVLGAEEGDGGVRLTVCDTGSGIPDYALGRIF
ERFYSLPRDNGQKSSGLGLAFVREVARLHQGDIELVNRPEGGVCALLRLHAHFT