Protein Info for BWI76_RS04150 in Klebsiella michiganensis M5al

Annotation: lipoate--protein ligase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 TIGR00545: lipoyltransferase and lipoate-protein ligase" amino acids 2 to 329 (328 residues), 448 bits, see alignment E=9.6e-139 PF03099: BPL_LplA_LipB" amino acids 53 to 157 (105 residues), 34.4 bits, see alignment E=1.9e-12 PF10437: Lip_prot_lig_C" amino acids 250 to 332 (83 residues), 62.1 bits, see alignment E=4.1e-21

Best Hits

Swiss-Prot: 91% identical to LPLA_CITK8: Lipoate-protein ligase A (lplA) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K03800, lipoate-protein ligase A [EC: 2.7.7.63] (inferred from 93% identity to enc:ECL_00797)

MetaCyc: 91% identical to lipoate--protein ligase A (Escherichia coli K-12 substr. MG1655)
RXN-17127 [EC: 6.3.1.20]; 6.3.1.20 [EC: 6.3.1.20]; 6.3.1.20 [EC: 6.3.1.20]

Predicted SEED Role

"Lipoate-protein ligase A" in subsystem Lipoic acid metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.63 or 6.3.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AY73 at UniProt or InterPro

Protein Sequence (338 amino acids)

>BWI76_RS04150 lipoate--protein ligase A (Klebsiella michiganensis M5al)
MSTLRLLLSDSYDPWFNLAVEESIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRR
MEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTVSTNIVLSALNALGVEAEASG
RNDLVVKTTEGDRKVSGSAYRETMDRGFHHGTLLLNADLSRLANYLNPDKKKLQAKGITS
VRGRVANLVELLPEITHQQVCEAIQEAFFAHYGERVAAEVISPENMPDLPNFAATFARQS
SWEWNFGQAPAFSHLLDERFTWGGVELHCDVEKGHITRTQVFTDSLNPAPLEALAARLQG
CLYRADMLQQECDALIGDFPEQENELRELSAWIAQAVR