Protein Info for BWI76_RS04135 in Klebsiella michiganensis M5al

Annotation: thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR02643: thymidine phosphorylase" amino acids 2 to 438 (437 residues), 776.3 bits, see alignment E=9e-238 PF02885: Glycos_trans_3N" amino acids 5 to 67 (63 residues), 66.3 bits, see alignment E=2.5e-22 TIGR02644: pyrimidine-nucleoside phosphorylase" amino acids 5 to 423 (419 residues), 555.2 bits, see alignment E=9e-171 PF00591: Glycos_transf_3" amino acids 79 to 310 (232 residues), 196.3 bits, see alignment E=1.1e-61 PF07831: PYNP_C" amino acids 350 to 423 (74 residues), 81.5 bits, see alignment E=4.4e-27

Best Hits

Swiss-Prot: 93% identical to TYPH_KLEP3: Thymidine phosphorylase (deoA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 92% identity to ecx:EcHS_A4617)

MetaCyc: 92% identical to thymidine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]; Thymidine phosphorylase. [EC: 2.4.2.2, 2.4.2.3, 2.4.2.4]

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.3

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AY98 at UniProt or InterPro

Protein Sequence (440 amino acids)

>BWI76_RS04135 thymidine phosphorylase (Klebsiella michiganensis M5al)
MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTVSEGQIAALAMTIFFHDMSMPERVSLT
MAMRDSGTVLDWKHLNLNGPIVDKHSTGGVGDVTSLMLGPMVAACGGYVPMISGRGLGHT
GGTLDKLEAIPGFDIFPDDSRFREIIKDVGVAIIGQTSSLAPADKRFYATRDITATVDSI
PLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSAALAEAIVGVSNGAGVRTTALLT
DMNQVLASSAGNAVEVREAVQFLTGEYRNPRLLEVTMALCVEMLISGKLAENEADARAKL
QAVLDNGKAADVFGRMVAAQKGPNDFVENYDKYLPAAMLSKAVYAEGEGFVAEMDTRALG
MAVVSMGGGRRQASDLIDYSVGFTDMVRLGARVDAQQPLAVIHAKDENSWQEAARAVKAA
IKLGAKAPEMTPTVYRRITE