Protein Info for BWI76_RS03855 in Klebsiella michiganensis M5al

Annotation: 5-carboxymethyl-2-hydroxymuconate Delta-isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02962: CHMI
  • amino acids 2 to 125 (124 residues), 174.6 bits, E=5.1e-56

Best Hits

Swiss-Prot: 82% identical to HPCD_ECOLX: 5-carboxymethyl-2-hydroxymuconate Delta-isomerase (hpcD) from Escherichia coli

KEGG orthology group: K01826, 5-carboxymethyl-2-hydroxymuconate isomerase [EC: 5.3.3.10] (inferred from 94% identity to esc:Entcl_3800)

MetaCyc: 82% identical to 5-carboxymethyl-2-hydroxymuconate isomerase (Escherichia coli B)
5-carboxymethyl-2-hydroxymuconate Delta-isomerase. [EC: 5.3.3.10]

Predicted SEED Role

"5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic amino acid degradation or Central meta-cleavage pathway of aromatic compound degradation (EC 5.3.3.10)

MetaCyc Pathways

• 4-hydroxyphenylacetate degradation (6/8 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Tyrosine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.3.10

Sequence Analysis Tools

See A0A285AY72 at UniProt or InterPro

Protein Sequence (126 amino acids)

>BWI76_RS03855 5-carboxymethyl-2-hydroxymuconate Delta-isomerase (Klebsiella michiganensis M5al)
MPHFIAECTDNIREQADLPDLFSKVNDALAATGIFPIGGIRSRAIWLDTWQMADGQHDYA
FVHMTLKIGAGRSLESRQDVGDMLFTLIKAHFAALMESRYLALSFELDELHPTLNYKQNN
VHALFK