Protein Info for BWI76_RS03830 in Klebsiella michiganensis M5al

Annotation: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 520 TIGR02310: 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component" amino acids 2 to 520 (519 residues), 1121.3 bits, see alignment E=0 PF11794: HpaB_N" amino acids 15 to 281 (267 residues), 310.2 bits, see alignment E=1.5e-96 PF03241: HpaB" amino acids 288 to 489 (202 residues), 219.5 bits, see alignment E=2.6e-69

Best Hits

Swiss-Prot: 98% identical to HPAB_KLEOX: 4-hydroxyphenylacetate 3-monooxygenase oxygenase component (hpaB) from Klebsiella oxytoca

KEGG orthology group: K00483, 4-hydroxyphenylacetate-3-hydroxylase large chain [EC: 1.14.13.3] (inferred from 97% identity to kpu:KP1_0734)

MetaCyc: 94% identical to 4-coumarate 3-monooxygenase (Escherichia coli W)
RXN-14988 [EC: 1.14.14.9]; 1.14.14.9 [EC: 1.14.14.9]; 1.14.14.9 [EC: 1.14.14.9]; 1.14.14.9 [EC: 1.14.14.9]

Predicted SEED Role

"4-hydroxyphenylacetate 3-monooxygenase (EC 1.14.13.3)" in subsystem 4-Hydroxyphenylacetic acid catabolic pathway or Aromatic Amin Catabolism (EC 1.14.13.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.3, 1.14.14.9

Use Curated BLAST to search for 1.14.13.3 or 1.14.14.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AY84 at UniProt or InterPro

Protein Sequence (520 amino acids)

>BWI76_RS03830 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component (Klebsiella michiganensis M5al)
MKPENFRADTKRPLTGEEYLKSLQDGREIYIYGERVKDVTTHPAFRNAAASVAQMYDALH
KPELQDTLCWGTDTGSGGYTHKFFRVAKSADDLRQQRDAIAEWSRLSYGWMGRTPDYKAA
FGCALGANPGFYGQFEQNARDWYTRIQETGLYFNHAIVNPPIDRHKPADEVKDVYIKLEK
ETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENPDFALMFVAPMDAEGVKLISRAS
YELVAGATGSPYDYPLSSRFDENDAILVMDNVLIPWENVLIYRDFDRCRRWTMEGGFARM
YPLQACVRLAVKLDFITALLKRSLECTGTLEFRGVQAELGEVVAWRNMFWALSDSMCAEA
TPWVNGAYLPDHAALQTYRVMAPMAYAKIKNIIERSVTSGLIYLPSSARDLNNPQINEYL
AKYVRGSNGMDHVERIKILKLMWDAIGSEFGGRHELYEINYSGSQDEIRLQCLRQAQSSG
NMDKMMAMVDRCLSEYDQNGWTVPHLHNNTDINMLDKLLK