Protein Info for BWI76_RS03675 in Klebsiella michiganensis M5al

Annotation: restriction modification system DNA specificity domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 PF01420: Methylase_S" amino acids 3 to 158 (156 residues), 64.9 bits, see alignment E=5.5e-22 amino acids 229 to 389 (161 residues), 41 bits, see alignment E=1.2e-14

Best Hits

Predicted SEED Role

"Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AY15 at UniProt or InterPro

Protein Sequence (439 amino acids)

>BWI76_RS03675 restriction modification system DNA specificity domain-containing protein (Klebsiella michiganensis M5al)
MGSKWKECELGDFIELKRGYDLPKSTRNEGSIPIISSSGFTDFHDKPMVKGPGVVTGRYG
TIGEVFYSEEDFWPLNTTLYVVDFKGNDPLFVYYLLQTISYADYTDKAAVPGVNRNHLHK
AKVKVPIYLDIQQKVAAQLYQLEKRVTLGKQINQTLEQMSQTLFKSWFVDFDPVIDNALD
AGNPIPEALQSRAELRQKVRSSADFKPLPADIRALFPAEFEETELGWVPKDWYHKNAEEI
ATISIGKTPPRNQKECFSHKKDSNYTWVSIKDLGNCNVFIKESSEYLTTDAVNNYNVKIV
PKGAVLLSFKLTIGRIAIAESILTTNEAIAHFYNMKHGVNKEYLYSYLQHFDYNTLGSTS
SIATAVNSKIIRKIPILLPDTDILHQYKISTDIIFKRISFNNRNTYDLTALRDTLLPKLI
SGELSLEDLPDLVNQTEPA