Protein Info for BWI76_RS03370 in Klebsiella michiganensis M5al

Annotation: type I restriction-modification system subunit S

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 PF01420: Methylase_S" amino acids 7 to 180 (174 residues), 25.8 bits, see alignment E=5.5e-10 amino acids 267 to 429 (163 residues), 54.6 bits, see alignment E=8.3e-19

Best Hits

Predicted SEED Role

"Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AXS9 at UniProt or InterPro

Protein Sequence (483 amino acids)

>BWI76_RS03370 type I restriction-modification system subunit S (Klebsiella michiganensis M5al)
MNENKLPREWVNTKLGNIVDYGRSAKKTLVDVTENTWVLELEDIEKDSSKLLSKVRVTDR
PFKSTKNCFNKGDVLYGKLRPYLNKVIMADDEGVCTTEIIPINAEPFCINKYIFFWLKSP
FFLRYVNEVSYGVNMPGLGTNDGRNAPFYLAPLAEQKIISNKLGSLWARVESIKSRLENI
PELLKKFRHSVLLSAVSGKLTAEWRNENKTSESAKELVVRWISLRKERFSIYQNELLALG
DLKRTKKFIEPHLPDIETGGELVVPPSWEIVSVSQIADCLDSQRIPVKKEKRIKSEGLYP
YFGANGEVDRVDDFIFNGDYVLVTEDETFYGRVKPIAYRYTGKCWVNNHVHILSAPTKSA
NDYLCYALMYYKIIPWLTGTTGRAKLTQAALNVLPIGLPPEKEIEEIVLRIDQFLSYADS
IQRAANNALARINNLTQSILAKAFCGELTAQWREENLDLISGINSAEALLQKISTEKLAS
GTS