Protein Info for BWI76_RS03020 in Klebsiella michiganensis M5al

Annotation: tRNA hydroxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 PF06175: MiaE" amino acids 5 to 248 (244 residues), 300.3 bits, see alignment E=3.4e-94

Best Hits

Swiss-Prot: 78% identical to MIAE_SALTY: tRNA-(ms[2]io[6]A)-hydroxylase (miaE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K06169, tRNA-(ms[2]io[6]A)-hydroxylase [EC: 1.-.-.-] (inferred from 95% identity to kva:Kvar_4593)

MetaCyc: 78% identical to tRNA 2-(methylsulfanyl)-N6-isopentenyl adenosine37 hydroxylase (Salmonella enterica enterica serovar Typhimurium str. LT2)
RXN-10751 [EC: 1.14.99.69]

Predicted SEED Role

"tRNA-(ms[2]io[6]A)-hydroxylase (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.- or 1.14.99.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AX13 at UniProt or InterPro

Protein Sequence (252 amino acids)

>BWI76_RS03020 tRNA hydroxylase (Klebsiella michiganensis M5al)
MDYPQILAPILGFLHCPTPQAWIDEARKPENLPLLLTDHMVCELKAAQNAMLLVRRYVAD
KDGADELLACLKPYEDFTYRWGPEPDFVALHKQINKSAMPQTDDPWGRQLLDSMILLIKE
ELHHFWQVREIMLSRDIPYVKITASNYARGLRREVRSHEPVMLIDKLICGAYIEARSCER
FAALAPWLDDDLQKFYLSLLRSEARHYQDYLDLAQRIAADDISERVRQLGEAEAALINAP
EAEFRFHSGVPV