Protein Info for BWI76_RS02950 in Klebsiella michiganensis M5al

Annotation: 6-phospho-beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 PF02056: Glyco_hydro_4" amino acids 5 to 185 (181 residues), 172.1 bits, see alignment E=9.2e-55 PF11975: Glyco_hydro_4C" amino acids 196 to 410 (215 residues), 209.1 bits, see alignment E=7.9e-66

Best Hits

Swiss-Prot: 50% identical to LICH_BACSU: Probable 6-phospho-beta-glucosidase (licH) from Bacillus subtilis (strain 168)

KEGG orthology group: K01222, 6-phospho-beta-glucosidase [EC: 3.2.1.86] (inferred from 88% identity to kpu:KP1_0528)

Predicted SEED Role

"6-phospho-beta-glucosidase (EC 3.2.1.86)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.86)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.86

Use Curated BLAST to search for 3.2.1.86

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWV9 at UniProt or InterPro

Protein Sequence (435 amino acids)

>BWI76_RS02950 6-phospho-beta-glucosidase (Klebsiella michiganensis M5al)
MQTLKIAVIGGGSSYTPELIEGIILRHQQIPVTELALVDVEAGREKVAIIAALAQRMLKR
HGLEQVKVSVHFRLDEAIRGAKFVLTQLRVGQLAARAADERLGLKYNLLGQETTGVGGFA
KALRTIPVMLQVARKVEALAPDAFILNFTNPAGIVTEAVSRYSSAKIIGLCNVPINMQHT
IAGMLEAKAEEVRLSFAGLNHMVWVHKVMQGREDVTRKVLDMLCDGQSLSMNNIKSLPWP
AEFLRALKAIPCPYHRYFWLTPAMLAEEIEAAKTKGTRAEQVMKVEQELFDIYARPELDE
KPEQLSLRGGAYYSEVAVELINAIYNNLGKEMVVNTRNNGAIHGFDDDAVVEINSLIDAQ
GARPLAFGALPPIMNGLTQQVKAFERLTIEAAVHGCRDSALLALVSNPLVGNVTDARALL
DEVLTINRPWLTQFN