Protein Info for BWI76_RS02890 in Klebsiella michiganensis M5al

Annotation: L-seryl-tRNA selenium transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01437: uncharacterized pyridoxal phosphate-dependent enzyme
  • amino acids 4 to 365 (362 residues), 606.3 bits, E=8.7e-187
• PF03841: SelA
  • amino acids 21 to 320 (300 residues), 49 bits, E=7e-17

Best Hits

KEGG orthology group: K01042, L-seryl-tRNA(Ser) seleniumtransferase [EC: 2.9.1.1] (inferred from 94% identity to kva:Kvar_4610)

Predicted SEED Role

"D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-)" (EC 4.3.1.-)

MetaCyc Pathways

• L-selenocysteine biosynthesis I (bacteria) (3/3 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Selenoamino acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.9.1.1

Use Curated BLAST to search for 2.9.1.1 or 4.3.1.-

Sequence Analysis Tools

See A0A285AX09 at UniProt or InterPro

Protein Sequence (372 amino acids)

>BWI76_RS02890 L-seryl-tRNA selenium transferase (Klebsiella michiganensis M5al)
MPSIFEKYDLKQVINTSGRMTILGVSTPRPEVVEAAMAGMNQYFEMKDLVNKTGAYIAKL
LDVEGATVVSCASAGIAQSVAAVLVKDSDWLLENLHVTPIENNEIVLPKGHNVNFGAPVG
TMVALGGGRLVEAGYANECSAGQLAAAITPRTAAILYIKSHHCVQKSMLNVAQAAAVAQR
HNLPLIVDAAAEEDLQAYYRAGADLVIYSGAKAIEGPTSGLVIGKTQYVEWVKRQSNGIG
RAMKVGKEGILGLTCAIEHYLTASKESGEQMVAKMTPFIQQLNTLNGVTARVVWDSAGRD
IARAEIKFDEAVTGVNTGDLVNALKQGEYAIYFRGYKANEGIIEADVRSVNAQQLEIVSR
RIAEVLNKEKNA