Protein Info for BWI76_RS02755 in Klebsiella michiganensis M5al

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02449: Glyco_hydro_42
  • amino acids 10 to 376 (367 residues), 275.1 bits, E=1.8e-85
• PF08532: Glyco_hydro_42M
  • amino acids 390 to 598 (209 residues), 108.2 bits, E=6.6e-35

Best Hits

KEGG orthology group: K12308, beta-galactosidase [EC: 3.2.1.23] (inferred from 90% identity to kpe:KPK_5057)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

• lactose degradation III (1/1 steps found)
• xyloglucan degradation II (exoglucanase) (4/8 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - ganglio series
• Other glycan degradation
• Sphingolipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

See A0A285AWR5 at UniProt or InterPro

Protein Sequence (669 amino acids)

>BWI76_RS02755 beta-galactosidase (Klebsiella michiganensis M5al)
MSDLFYGVAYYDEYMPEDRLAKDIALMQETGINVVRIAESTWSTLEPREGEYNFYHIDRV
LDAMHEAGIAVIIGTPTYAVPAWLAAKHPDILVTTVDGQQKYGPRQIMDIVNPTFRRYAE
KIIRTLMAHVQRHPAIIGWQLDNETKHYDNIGRYMQEGFVRSLREKYPDLDRLNNDFGLD
YWSNRIDSWQDFPPVENTINASLACAFSRYQRQQVTEYLAWQAEIVREYAQPHQFVTHNF
DFEWRGYSYGVQPRVDHFAAAQALDIAGVDIYHPSQKHLTGREIAFGGAITRSLKQGNNY
FVLETQAQGFAQWTPYPGQLRLQAFSHVASGAAMVSYWHWHSIHNAFETYWKGLLSHDFS
RNATWQEATTIGADFARLSPQLAELKAENDVALLISNEAMDALNQFRPGDAQGNIYNDIF
RRFHDALYDHNISLDIIHDVNEETAHYRVLIVPGLYAADDGLLTRINRYIEQGGRALIGF
KSGFSDENVKVRSGAQPGVLRESCGVSYSQFTLPEETTVSACSADIDCSKDNQAELWMEL
LTPDAGTRTLLHYQHPAWSDFAAATEADYGQGRAIYVGFLPQKTLISQLFDRLTAGLDLR
SRTSAYRYPLVVKKMRNRDGNSIYFLFNYSGDPLAFISETSGNALLSGEKVHCGQPLRLR
AWEFSIIES