Protein Info for BWI76_RS02730 in Klebsiella michiganensis M5al

Annotation: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR01390: 2',3'-cyclic-nucleotide 2'-phosphodiesterase" amino acids 22 to 646 (625 residues), 1187.8 bits, see alignment E=0 PF00149: Metallophos" amino acids 24 to 260 (237 residues), 57.2 bits, see alignment E=3.3e-19 PF02872: 5_nucleotid_C" amino acids 370 to 550 (181 residues), 71.8 bits, see alignment E=7.1e-24

Best Hits

Swiss-Prot: 89% identical to CPDB_SALTY: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (cpdB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] (inferred from 89% identity to cko:CKO_03616)

MetaCyc: 86% identical to 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (Escherichia coli K-12 substr. MG1655)
2',3'-cyclic-nucleotide 2'-phosphodiesterase. [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3'-nucleotidase. [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.6 or 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWR0 at UniProt or InterPro

Protein Sequence (647 amino acids)

>BWI76_RS02730 2',3'-cyclic-nucleotide 2'-phosphodiesterase (Klebsiella michiganensis M5al)
MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKDSATEKFGLVRTATLIE
KARQEAKNSVLVDNGDVIQGSPLGDYMAAKGLKEGDVHPVYKAMNTLDYAVGNLGNHEFN
YGLDFLHKALAGAKFPYVNANIIDAKTGKPMFTPYLIQDTQVQDSDGKTQTLRIGYIGFV
PPQIMTWDKANLSGKVTVNDITATARKFVPEMREKGADVVVVIAHSGLSADPYQAMAENS
VYYLSQVPGVDAIMFGHAHAVFPSKDFAGIKGVDIAKGTLNGVPAVMPGMWGDHLGVVDL
VLNNDSGKWQVSAAKAEARPIYDAAAKKSLAAEDSNMVAVLKADHDATREFVGKPIGQSS
DNMYSYLALVQDDPTVQVVNMAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPAS
FVEVEKGQLTFRNAADLYLYPNTLVVMKVSGKEVKEWLECSAGQFNQIDPASSKPQSLIN
WDGFRTYNFDVIDGVNYQIDITQPARYDGECNSVNPQAERIKHLTFNGKPIDANATFLVA
TNNYRAYGGKFQGTGEDHIAFASPDENRSVLAAWIGAESKKNGEIHPAADNNWRLAPIHS
SVPLDIRFETSPGDKAAAFIKEKAQYPMRQVATDDIGFAIYQLDLSQ