Protein Info for BWI76_RS02590 in Klebsiella michiganensis M5al

Annotation: isovaleryl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 545 PF18158: AidB_N" amino acids 10 to 167 (158 residues), 190.8 bits, see alignment E=3.2e-60 PF02770: Acyl-CoA_dh_M" amino acids 181 to 274 (94 residues), 55.9 bits, see alignment E=7.8e-19 PF00441: Acyl-CoA_dh_1" amino acids 285 to 439 (155 residues), 99.9 bits, see alignment E=3.3e-32 PF08028: Acyl-CoA_dh_2" amino acids 311 to 420 (110 residues), 37.9 bits, see alignment E=4.2e-13

Best Hits

Swiss-Prot: 79% identical to AIDB_ECOLI: Putative acyl-CoA dehydrogenase AidB (aidB) from Escherichia coli (strain K12)

KEGG orthology group: K09456, putative acyl-CoA dehydrogenase (inferred from 92% identity to kpn:KPN_04580)

MetaCyc: 79% identical to isovaleryl-CoA dehydrogenase and DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
RXN0-2301 [EC: 1.3.8.4]

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.4 or 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWM6 at UniProt or InterPro

Protein Sequence (545 amino acids)

>BWI76_RS02590 isovaleryl-CoA dehydrogenase (Klebsiella michiganensis M5al)
MHWQTHTVFNQPSPLCNSNLFLSDTALREAVVREGAGWDGDLLASIGQQLGTAESLELGR
LANSNPPELLRYDATGSRLDDVRFHPAWHLLMQGLCANRVHNLAWQEDAREGAFVARAAR
FMLHAQVEAGTLCPITMTFAAIPLLQRSLPRPFSDWLSPLLSDRYDPHLIPGNQKRGLLI
GMGMTEKQGGSDVLSNTTRAEKTAEGFYHLIGHKWFFSVPQSDAHLVLAQAPAGLSCFFV
PRLLPDGQRNGVHLERLKDKLGNRANASSEVEFFNACGWLVGEEGDGVRQILKMGGLTRF
DCALGSHALMRRAYSVALYHALQRQAFGKNLVDQPMMRQVLGQMALRLEGQTAFLFRLAR
AWDRRDDAQEALWARLFTPAAKFAICKSGIAFVAEAMEVLGGSGYCEESELPRLYREMPV
NSIWEGSGNIMCLDVMRVLMKQPAALELLAAECAEVKGQNRHFDRAWRQLQQWLRRPLEE
QGREITRLVYLLGVGAQVLRFASPPLAEAWCRMMLDTRGGMRLDEQTLDDLLLRAMGRGR
QAPQA