Protein Info for BWI76_RS02545 in Klebsiella michiganensis M5al

Annotation: protease modulator HflK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 418 transmembrane" amino acids 79 to 100 (22 residues), see Phobius details PF12221: HflK_N" amino acids 1 to 60 (60 residues), 60.1 bits, see alignment E=1.5e-20 TIGR01933: HflK protein" amino acids 97 to 356 (260 residues), 411.4 bits, see alignment E=7.4e-128 PF01145: Band_7" amino acids 99 to 270 (172 residues), 112.8 bits, see alignment E=2e-36

Best Hits

Swiss-Prot: 89% identical to HFLK_ECOLI: Modulator of FtsH protease HflK (hflK) from Escherichia coli (strain K12)

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 94% identity to kva:Kvar_4682)

MetaCyc: 89% identical to regulator of FtsH protease (Escherichia coli K-12 substr. MG1655)
3.4.24.-

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWQ5 at UniProt or InterPro

Protein Sequence (418 amino acids)

>BWI76_RS02545 protease modulator HflK (Klebsiella michiganensis M5al)
MAWNQPGNNGQDRDPWGSSNNQGGNSGGNGNKGGRDQGPPDLDDIFRKLSKKLGGLGGGK
GGLGGGSGSQGPRGPMGGRIVGIVAAAAVIIWAASGFYTIKEAERGVVTRFGKFSHLVEP
GLNWKPTFIDSVQAVNVESVRELAASGVMLTSDENVVRVEMNVQYRVTDPERYLFSVTSA
DDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIRPYNMGITLLDVNFQT
ARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKTQTVLE
AQGEVARFAKILPEYKAAPEITRERLYIETMEKVLSHTRKVLVNDKGSNLMVLPLDQMLK
GAGAPAAKSDSSGASDLLRLPPASSSNSASNASTSTGGTIMDQRRANAQRNDYQRQGE