Protein Info for BWI76_RS02540 in Klebsiella michiganensis M5al

Annotation: GTPase HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 10 to 361 (352 residues), 511.7 bits, E=4.3e-158
• PF13167: GTP-bdg_N
  • amino acids 25 to 112 (88 residues), 109.6 bits, E=2.3e-35
• PF16360: GTP-bdg_M
  • amino acids 114 to 192 (79 residues), 103.7 bits, E=1.6e-33
• PF02421: FeoB_N
  • amino acids 199 to 336 (138 residues), 32 bits, E=2.1e-11
• PF01926: MMR_HSR1
  • amino acids 200 to 318 (119 residues), 75.3 bits, E=1e-24
• PF19275: HflX_C
  • amino acids 340 to 407 (68 residues), 33.3 bits, E=1e-11

Best Hits

Swiss-Prot: 93% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 97% identity to kpe:KPK_5097)

MetaCyc: 93% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A285AWL5 at UniProt or InterPro

Protein Sequence (426 amino acids)

>BWI76_RS02540 GTPase HflX (Klebsiella michiganensis M5al)
MFDRYDAGEQAVLVHIYFSQDKDMEDLQEFETLASSAGVEAMQVITGSRKAPHPKYFVGE
GKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVIDRTGLILDIFAQRARTHEG
KLQVELAQLRHMATRLVRGWTHLERQKGGIGLRGPGETQLETDRRLLRNRIMQILARLEK
VEKQREQGRRSRAKADIPTVSLVGYTNAGKSTLFNQITEAEVYAANQLFATLDPTLRRID
VPDVGETVLADTVGFIRHLPHDLVAAFKATLQETRQATLLLHIIDAADVRVQENIDAVNT
VLAEIEADEIPTLLVMNKIDMLDDFEPRIDRDEENKPIRVWLSAQTGVGVPLLFQALTER
LSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKEWMEDDGSVGLQVRMPIVDWRRLCKQEPA
LVEYVI