Protein Info for BWI76_RS02530 in Klebsiella michiganensis M5al

Annotation: tRNA dimethylallyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 TIGR00174: tRNA dimethylallyltransferase" amino acids 12 to 300 (289 residues), 407.2 bits, see alignment E=1.8e-126 PF01715: IPPT" amino acids 46 to 288 (243 residues), 306.1 bits, see alignment E=2.1e-95

Best Hits

Swiss-Prot: 98% identical to MIAA_KLEP7: tRNA dimethylallyltransferase (miaA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 98% identity to kpn:KPN_04569)

MetaCyc: 91% identical to tRNA dimethylallyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-6274 [EC: 2.5.1.75]

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWL8 at UniProt or InterPro

Protein Sequence (316 amino acids)

>BWI76_RS02530 tRNA dimethylallyltransferase (Klebsiella michiganensis M5al)
MSDVTEASLPKAIFLMGPTASGKTALAIALRKVLPVELISVDSALIYRGMDIGTAKPDAA
ELSAAPHRLLDILDPAEAYSAADFRRDALAEMADIVAAGRIPLLVGGTMLYFKALLEGLS
PLPSADPEVRARIEQQAAEQGWNALHQQLQEIDPVAAARIHPNDPQRLSRALEVFFISGK
TLTELTQTSGDALPYKVHQFAIAPASRELLHQRIEQRFHQMLASGFEAEVRALFARGDLH
TDMPSIRCVGYRQMWSYLNGEIPYDEMVYRGVCATRQLAKRQVTWLRGWEGIHWLDSEQP
EQAFNKVLQVVGASLD