Protein Info for BWI76_RS02475 in Klebsiella michiganensis M5al
Annotation: ribosome small subunit-dependent GTPase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to RSGA_KLEP3: Small ribosomal subunit biogenesis GTPase RsgA (rsgA) from Klebsiella pneumoniae (strain 342)
KEGG orthology group: None (inferred from 91% identity to eae:EAE_09265)MetaCyc: 88% identical to ribosome small subunit-dependent GTPase A (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]
Predicted SEED Role
"Ribosome small subunit-stimulated GTPase EngC" in subsystem Universal GTPases
MetaCyc Pathways
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
- pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
- pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- UTP and CTP dephosphorylation II (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.6.1.15
Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AWK9 at UniProt or InterPro
Protein Sequence (353 amino acids)
>BWI76_RS02475 ribosome small subunit-dependent GTPase (Klebsiella michiganensis M5al) MSKNKLSKGQQRRVKANHQRRLKTTSEKADFDDNLFGEKSEGTVISRFGMHADVESADGS VHRCNIRRTIRSLVTGDRVVWRPGKSEADGVNVKGIVEAVHERTSVLTRPDFYDGVKPIA ANINQIVIVSAILPELSPNIIDRYLVACEALNVEPLIVLNKIDLLDQESMAFVNEQMDIY RHIGYRVLMVSSRTQDGLKPLEEALTDRISIFAGQSGVGKSSLLNALLGLHDDQILTNDV SDVSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLWHLDAEQITHGFVEFHDYLGRCKY RDCKHDADPGCAIREAVEKGEIAESRFENYHRILESMAQVQVKTRKSFSSSDD