Protein Info for BWI76_RS02375 in Klebsiella michiganensis M5al

Annotation: secretion protein HlyD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 transmembrane" amino acids 22 to 50 (29 residues), see Phobius details PF13533: Biotin_lipoyl_2" amino acids 69 to 116 (48 residues), 29.4 bits, see alignment 1.4e-10 PF00529: CusB_dom_1" amino acids 79 to 401 (323 residues), 37.1 bits, see alignment E=6.3e-13 PF16576: HlyD_D23" amino acids 256 to 340 (85 residues), 32 bits, see alignment E=2e-11 PF13437: HlyD_3" amino acids 263 to 351 (89 residues), 39.9 bits, see alignment E=1.5e-13

Best Hits

Swiss-Prot: 41% identical to MCHE_ECOLX: Microcin H47 secretion protein MchE (mchE) from Escherichia coli

KEGG orthology group: K02022, (no description) (inferred from 77% identity to kva:Kvar_4714)

Predicted SEED Role

"FIG00732435: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>BWI76_RS02375 secretion protein HlyD (Klebsiella michiganensis M5al)
MHNDLYRKEAIEYKRFYWKGKALLLAGLPAWLVTLLSLIFLIALVATIIFCKFTQRIDVR
GEVITLPHSINVFSPQQGFVVTQYVKVGEIVKKGQPLYELDVSRNTTSGNVSESQIESIN
EKIINANDIIKKLKKNKDETLNALDSQIKNVKDSLKETNHMLANTQAGLRKMYENLSNYD
KYLKNGLITKDQYNYQHSLYFQQQSNYQSLISQQMQLDTQVTQINSDRVTKAADFDNQIS
NQYNQINDYKNQLIESNASGNVVIKATANGKVESLAVTKGQMVENGSSLAQIKPTGDVEY
YLILWLPNNSIPYIKPGDTINIRYDAFPADKFGQFPGKVLSISSMPASRQELSEYTNVSN
GTNQQELALYKAIVTIKHKSFNYKGKTLTLSNGLKAQAVVFLEERPLYMWMFTPFYKISQ
SVSGPIND