Protein Info for BWI76_RS02355 in Klebsiella michiganensis M5al

Annotation: molecular chaperone GroEL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 TIGR02348: chaperonin GroL" amino acids 3 to 526 (524 residues), 849.5 bits, see alignment E=4.4e-260 PF00118: Cpn60_TCP1" amino acids 23 to 522 (500 residues), 269.5 bits, see alignment E=2.8e-84

Best Hits

Swiss-Prot: 98% identical to CH60_KLEAK: 60 kDa chaperonin (groL) from Klebsiella aerogenes (strain ATCC 13048 / DSM 30053 / JCM 1235 / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006)

KEGG orthology group: K04077, chaperonin GroEL (inferred from 98% identity to enc:ECL_00541)

MetaCyc: 96% identical to chaperonin GroEL (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Heat shock protein 60 family chaperone GroEL" in subsystem GroEL GroES or Staphylococcal pathogenicity islands SaPI

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWK1 at UniProt or InterPro

Protein Sequence (548 amino acids)

>BWI76_RS02355 molecular chaperone GroEL (Klebsiella michiganensis M5al)
MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREI
ELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIIAEGLKAVAAGMNPMDLKRGI
DKAVVAAVEELKTLSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDG
TGLEDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAV
AKAGKPLVIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTV
ISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVTQIRKQIEEATSDY
DREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVDDALHATRAAVEEGVVAGGGVALV
RVAAKLAGLTAQNEDQNVGIKVALRAMEAPLRQIVSNAGEEPSVVANNVKAGEGNYGYNA
ATEEYGNMIDFGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKGDAPDLGAAGGMGG
MGGMGGMM