Protein Info for BWI76_RS02330 in Klebsiella michiganensis M5al

Annotation: aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00839: aspartate ammonia-lyase
  • amino acids 6 to 474 (469 residues), 873 bits, E=2.7e-267
• PF00206: Lyase_1
  • amino acids 13 to 345 (333 residues), 362.1 bits, E=2.9e-112
• PF10415: FumaraseC_C
  • amino acids 411 to 464 (54 residues), 63.4 bits, 2.1e-21

Best Hits

Swiss-Prot: 91% identical to ASPA_ECOLI: Aspartate ammonia-lyase (aspA) from Escherichia coli (strain K12)

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 97% identity to kpn:KPN_04529)

MetaCyc: 91% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

• superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
• L-aspartate degradation II (aerobic) (3/3 steps found)
• L-aspartate degradation III (anaerobic) (3/3 steps found)
• L-glutamate degradation II (2/2 steps found)
• L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.1

Sequence Analysis Tools

See A0A285AWH3 at UniProt or InterPro

Protein Sequence (478 amino acids)

>BWI76_RS02330 aspartate ammonia-lyase (Klebsiella michiganensis M5al)
MLNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAAQ
ANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVFQGGAGTSVNMNTNEVLANIGLE
LMGHQKGEYQHLNPNDHVNKCQSTNDAYPTGFRIAVYASILKLIDAINQLSEGFQQKAVE
FQDILKMGRTQLQDAVPMTLGQEFHAFNVLLNEETKCILRTAELLLEVNLGATAIGTRLN
TPDGYQQLAVQKLAEVSNLPVVPAEDLIEATSDCGAYVMVHSSLKRLAVKLSKICNDLRL
LSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTMASEAGQLQ
LNVMEPVIGQAMFESIHILTNACYNLLEKCVSGITANKAVCESYVYNSIGIVTYLNPYIG
HHNGDIVGKICAETGKSVRDVVLERGLLTAAELDDIFSAQNLMHPAYKAKRYTDESEQ