Protein Info for BWI76_RS02315 in Klebsiella michiganensis M5al

Annotation: protein-disulfide reductase DsbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 188 to 215 (28 residues), see Phobius details amino acids 228 to 252 (25 residues), see Phobius details amino acids 264 to 288 (25 residues), see Phobius details amino acids 309 to 338 (30 residues), see Phobius details amino acids 350 to 369 (20 residues), see Phobius details amino acids 380 to 399 (20 residues), see Phobius details amino acids 409 to 428 (20 residues), see Phobius details PF11412: DsbD_N" amino acids 29 to 137 (109 residues), 123.8 bits, see alignment E=1.2e-39 PF13386: DsbD_2" amino acids 191 to 380 (190 residues), 31 bits, see alignment E=7.1e-11 PF02683: DsbD" amino acids 192 to 397 (206 residues), 73.1 bits, see alignment E=8.9e-24 PF13899: Thioredoxin_7" amino acids 482 to 556 (75 residues), 50.8 bits, see alignment E=4.5e-17 PF13098: Thioredoxin_2" amino acids 484 to 580 (97 residues), 46.5 bits, see alignment E=1.2e-15

Best Hits

Swiss-Prot: 82% identical to DSBD_KLEP7: Thiol:disulfide interchange protein DsbD (dsbD) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 83% identity to kva:Kvar_4725)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWL3 at UniProt or InterPro

Protein Sequence (585 amino acids)

>BWI76_RS02315 protein-disulfide reductase DsbD (Klebsiella michiganensis M5al)
MAQRIITLILLLCSTSAIAGLFDAPGRSNFVPADQAFAFDFQQNQHDLNLTWQIKDGYYL
YRKQIKLTSADAAFSEPSLPAGEWHEDEFYGKSEIYRQRLTVPVTLTHAAAGATLTVTWQ
GCADAGFCYPPETKIVPLGNALAQSGDKKPMLNPPLPLETRPALPVESPADLAPPATDEK
SADLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGQKRLSTARALLLAFIYVQGMAL
TYTALGLVVAAAGLQFQAALQHPYVLIGLSVVFILLALSMFGLFTLQLPSSLQTRLTLMS
NKRQGGSTGGVFAMGAIAGLICSPCTTAPLSAILLYIAQSGNLWLGGGTLYLYALGMGLP
LILVTVFGNRLLPKSGPWMAHVKTAFGFVILALPVFLLERIVGEPWGLRLWSLLGVAFFS
WAFITSLGGTRPWLRIVQIAMLGAALVSARPLQDWAFDAPVAQQQAHLQFTRIHNVDELN
QALAQAKGKTVMLDLYADWCVACKEFEKYTFSSPDVQQALKETVLLQVDVTKNSEQDAAL
LKHLQVLGLPTILFFNERGEEQPAQRVTGFMDAAAFSAHLRDRQP