Protein Info for BWI76_RS02220 in Klebsiella michiganensis M5al

Annotation: carbon-phosphorus lyase subunit PhnH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 194 PF05845: PhnH" amino acids 5 to 192 (188 residues), 201 bits, see alignment E=7.9e-64 TIGR03292: phosphonate C-P lyase system protein PhnH" amino acids 7 to 190 (184 residues), 192.5 bits, see alignment E=2.8e-61

Best Hits

Swiss-Prot: 79% identical to PHNH_ECOLI: Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (phnH) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 92% identity to eae:EAE_08720)

MetaCyc: 79% identical to carbon-phosphorus lyase core complex subunit PhnH (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PhnH protein" in subsystem Alkylphosphonate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AWK2 at UniProt or InterPro

Protein Sequence (194 amino acids)

>BWI76_RS02220 carbon-phosphorus lyase subunit PhnH (Klebsiella michiganensis M5al)
MTLQPAFTLAVQDAQHSFRRLLKAMSEPGVIVSLQQLQHGWQPLNVASTSLLLTLVDHDT
PVWLAPALHNDLVGQNLRFHTGAPLVEQPQQAIFAVASDTISAEQLNVLSAGTVVAPETG
VTLVVQLASLSGGRMLRLTGAGIAEERMIAPQLPECIIEELTERPHPFPLGIDLILTCGE
RLLAIPRATHVEVC