Protein Info for BWI76_RS02115 in Klebsiella michiganensis M5al

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 715 PF04879: Molybdop_Fe4S4" amino acids 1 to 54 (54 residues), 60.7 bits, see alignment 1.6e-20 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 6 to 685 (680 residues), 1067.7 bits, see alignment E=0 PF00384: Molybdopterin" amino acids 61 to 487 (427 residues), 325.5 bits, see alignment E=6.4e-101 PF01568: Molydop_binding" amino acids 576 to 682 (107 residues), 93.7 bits, see alignment E=1.1e-30

Best Hits

Swiss-Prot: 95% identical to FDHF_ECOLI: Formate dehydrogenase H (fdhF) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 95% identity to eco:b4079)

MetaCyc: 95% identical to formate dehydrogenase H (Escherichia coli K-12 substr. MG1655)
RXN0-3281 [EC: 1.17.98.4]; FHLMULTI-RXN [EC: 1.17.98.4]

Predicted SEED Role

"Formate dehydrogenase H (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.98.4 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (715 amino acids)

>BWI76_RS02115 formate dehydrogenase subunit alpha (Klebsiella michiganensis M5al)
MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLKGYYGWDFINDTQILTP
RLKTPMIRRQRGGKLESVSWDEALDYVATRLSAIKEKYGPDAIQTTGSSRGTGNETNYVM
QKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNAITEIDNTDLVFIFGYNPADS
HPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIALKNGSNIALLNAIGHVIIEEDLY
DKSFVASRSEGFEEYRKIVEGYTPESVEEITGVSAQEIRACARMYANAKSAAILWGMGVT
QFYQGVETVRSLTSLAILTGNLGKPSVGVNPVRGQNNVQGACDMGALPDTYPGYQYVKFP
ENREKFAKAWGVESLPEHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFD
DLELVIVQDIFMTKTASAADVILPSTSWGEHEGVYTAADRGFQRFFKAVEPKWDLKTDWQ
IISEIATRMGYKMHYNNTQEIWDELRHLCPDFYGATYEKMGELGYVMWPCRDESDADQGT
SYLFKEKFDTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAA
LADEPGYAQINTADAERLGIEDEALVWVNSRKGKIITRAQVSDRPNKGAVYMTYQWWIGA
CNELVSENLSPITKTPEYKYCAVNIEPIADQRAAEQYVIDEYNKLKTRLRESALG