Protein Info for BWI76_RS01920 in Klebsiella michiganensis M5al
Annotation: alanine racemase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 91% identical to ALR1_ECOL6: Alanine racemase, biosynthetic (alr) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
KEGG orthology group: K01775, alanine racemase [EC: 5.1.1.1] (inferred from 93% identity to kva:Kvar_4808)MetaCyc: 90% identical to alanine racemase 1 (Escherichia coli K-12 substr. MG1655)
Alanine racemase. [EC: 5.1.1.1, 5.1.1.10]
Predicted SEED Role
"Alanine racemase, biosynthetic (EC 5.1.1.1)" in subsystem Alanine biosynthesis or Pyruvate Alanine Serine Interconversions (EC 5.1.1.1)
MetaCyc Pathways
- L-alanine degradation I (2/2 steps found)
- alanine racemization (1/1 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (1/6 steps found)
- L-lysine degradation V (2/9 steps found)
- ansatrienin biosynthesis (1/9 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
- superpathway of L-lysine degradation (12/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Cysteine metabolism
- D-Alanine metabolism
- D-Arginine and D-ornithine metabolism
- D-Glutamine and D-glutamate metabolism
- Glycine, serine and threonine metabolism
Isozymes
Compare fitness of predicted isozymes for: 5.1.1.1
Use Curated BLAST to search for 5.1.1.1 or 5.1.1.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AWD7 at UniProt or InterPro
Protein Sequence (359 amino acids)
>BWI76_RS01920 alanine racemase (Klebsiella michiganensis M5al) MQAATVVINRRALRHNLQRLRELAPASKLVAVVKANAYGHGLLETARTLPDADAFGVARL EEALRLRAGGIAQPILLLEGFFEAADLPVISTQRLHTAVHSPEQLAALEEADLPEPVTVW MKLDTGMHRLGVLPEQAEAFYQRLSQCKNVRQPVNVVSHFARADEPECGATERQLDIFTT FTEGKPGLRSIAASGGILLWPQSHFDWARPGIILYGVSPLDGGSTGADFGCQPVMSLTSS LIAVREHKAGEPVGYGGTWISERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGR VAMDMICVDLGPEAQDKSGDPVVLWGEGLPVERIAAITKVSAYELITRLTSRVSMKYVD