Protein Info for BWI76_RS01840 in Klebsiella michiganensis M5al

Updated annotation (from data): maltose ABC transporter, ATPase component (MalK)
Rationale: Specifically important for maltose utilization
Original annotation: ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 PF00005: ABC_tran" amino acids 20 to 161 (142 residues), 119.4 bits, see alignment E=2.8e-38 PF17912: OB_MalK" amino acids 235 to 288 (54 residues), 47.5 bits, see alignment 4.2e-16 PF08402: TOBE_2" amino acids 281 to 353 (73 residues), 51.1 bits, see alignment E=1.8e-17

Best Hits

Swiss-Prot: 95% identical to MALK_SALCH: Maltose/maltodextrin import ATP-binding protein MalK (malK) from Salmonella choleraesuis (strain SC-B67)

KEGG orthology group: None (inferred from 99% identity to eae:EAE_08395)

MetaCyc: 94% identical to maltose ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-16-RXN [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]

Predicted SEED Role

"Maltose/maltodextrin transport ATP-binding protein MalK (EC 3.6.3.19)" in subsystem Maltose and Maltodextrin Utilization (EC 3.6.3.19)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.19

Use Curated BLAST to search for 3.6.3.19 or 7.5.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AW85 at UniProt or InterPro

Protein Sequence (369 amino acids)

>BWI76_RS01840 maltose ABC transporter, ATPase component (MalK) (Klebsiella michiganensis M5al)
MASVQLRNVTKAWGDVVVSKDINLEIQDGEFVVFVGPSGCGKSTLLRMIAGLETVTSGDL
LIGDTRMNDVPPAERGIGMVFQSYALYPHLSVAENMSFGLKLAGAKKELINQRVTQVAEV
LQLAHLLERKPKALSGGQRQRVAIGRTLVAEPRVFLLDEPLSNLDAALRVQMRIEISRLH
KRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKPLELYHYPADRFVAGFIGSPKMN
FLPVKVTATAIEQVQVELPNRQQVWLPVDSARVQVGANMSLGIRPEHLLPSDIADVTLEG
EVQVVEQLGHETQIHIQIPSIRQNLVYRQNDVVLVEEGATFAIGLPPERCHLFREDGTAC
RRLHKEPGV