Protein Info for BWI76_RS01830 in Klebsiella michiganensis M5al

Updated annotation (from data): maltose ABC transporter, substrate-binding component MalE
Rationale: Specifically important for utilizing D-Maltose monohydrate.
Original annotation: maltose ABC transporter substrate-binding protein MalE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01547: SBP_bac_1" amino acids 46 to 309 (264 residues), 148.9 bits, see alignment E=3.4e-47 PF13416: SBP_bac_8" amino acids 50 to 344 (295 residues), 131.4 bits, see alignment E=5.6e-42

Best Hits

Swiss-Prot: 97% identical to MALE_KLEAE: Maltose/maltodextrin-binding periplasmic protein (malE) from Klebsiella aerogenes

KEGG orthology group: K10108, maltose/maltodextrin transport system substrate-binding protein (inferred from 97% identity to kpe:KPK_5253)

MetaCyc: 94% identical to maltose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-16-RXN [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]

Predicted SEED Role

"Maltose/maltodextrin ABC transporter, substrate binding periplasmic protein MalE" in subsystem Bacterial Chemotaxis or Maltose and Maltodextrin Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AW72 at UniProt or InterPro

Protein Sequence (396 amino acids)

>BWI76_RS01830 maltose ABC transporter, substrate-binding component MalE (Klebsiella michiganensis M5al)
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK
VSVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEISPDKAFQDKLYPFTW
DAVRYNGKLIAYPVAVEALSLIYNKDLVPNPPKTWEEIPALDKELKAKGKSALMFNLQEP
YFTWPLIAADGGYAFKFENGKYDVKNVGVDSAGAKAGLTFLVDLIKNKHMNADTDYSIAE
AAFNKGETAMTINGPWAWSNIDKSKVNYGVTLLPTFKGNASKPFVGVLSAGINAASPNKE
LAKEFLENYLMTDQGLDEVNKDKPLGAVALKSFQEKLEKDPRIAATMANAQKGEIMPNIP
QMSAFWYAVRTAVINAASGRQTVEAALKDAQSRITK