Protein Info for BWI76_RS01500 in Klebsiella michiganensis M5al

Annotation: DNA-directed RNA polymerase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1342 TIGR02013: DNA-directed RNA polymerase, beta subunit" amino acids 8 to 1339 (1332 residues), 1981.3 bits, see alignment E=0 PF04563: RNA_pol_Rpb2_1" amino acids 27 to 499 (473 residues), 137.4 bits, see alignment E=1.5e-43 PF04561: RNA_pol_Rpb2_2" amino acids 151 to 225 (75 residues), 32.1 bits, see alignment 2.9e-11 amino acids 338 to 454 (117 residues), 59.9 bits, see alignment E=8.6e-20 PF04565: RNA_pol_Rpb2_3" amino acids 513 to 580 (68 residues), 102.6 bits, see alignment 3.2e-33 PF10385: RNA_pol_Rpb2_45" amino acids 591 to 656 (66 residues), 100 bits, see alignment 1.9e-32 PF00562: RNA_pol_Rpb2_6" amino acids 717 to 1264 (548 residues), 442.7 bits, see alignment E=3.5e-136 PF04560: RNA_pol_Rpb2_7" amino acids 1266 to 1341 (76 residues), 100.4 bits, see alignment 1.9e-32

Best Hits

KEGG orthology group: K03043, DNA-directed RNA polymerase subunit beta [EC: 2.7.7.6] (inferred from 85% identity to aap:NT05HA_1813)

MetaCyc: 97% identical to RNA polymerase subunit beta (Escherichia coli K-12 substr. MG1655)
DNA-directed RNA polymerase. [EC: 2.7.7.6]

Predicted SEED Role

"DNA-directed RNA polymerase beta subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AW51 at UniProt or InterPro

Protein Sequence (1342 amino acids)

>BWI76_RS01500 DNA-directed RNA polymerase subunit beta (Klebsiella michiganensis M5al)
MVYSYTEKKRIRKDFGKRPQVLDIPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIK
SYSGNSELQYVSYRLGEPVFDVKECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQ
EVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYR
GSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVVFEIRDNKLQME
LVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDIKHIEVPVEYIAGKVASKD
YVDESTGELICPANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYMSETVRVDPTNDRL
SALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLRDEIEGSGIL
SKEDIIEVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLS
LGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTR
ERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGIVT
DEIHYLSAIEEGNYVIAQANSNLDDEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQV
VSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKR
GGTVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPIERG
DVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVS
RDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEK
LLRAIFGEKASDVKDSSLRVPNGVSGTIIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDL
SEELQILEAGLFGRIYAVLVSGGIEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRQIQPGDKMAGRHGNKGVISKIN
PIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGEKINAMLKQQQEVAK
LREFIQRAYDLGTDVRQKVDLNTFTDEEVMRLAENLKKGMPIATPVFDGAKESEIKELLQ
LGGLPTSGQITLFDGRTGEQFERQVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLG
GKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE