Protein Info for BWI76_RS01350 in Klebsiella michiganensis M5al

Annotation: NAD(P)H-flavin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 90% identical to FRE_SALTY: NAD(P)H-flavin reductase (fre) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K05368, aquacobalamin reductase / NAD(P)H-flavin reductase [EC: 1.16.1.3 1.5.1.29] (inferred from 94% identity to kpu:KP1_0201)

MetaCyc: 90% identical to FMN reductase [NAD(P)H] (Salmonella enterica enterica serovar Typhimurium)
FMNREDUCT-RXN [EC: 1.5.1.39]

Predicted SEED Role

"NAD(P)H-flavin reductase (EC 1.5.1.29) (EC 1.16.1.3)" in subsystem Ubiquinone Biosynthesis (EC 1.16.1.3, EC 1.5.1.29)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
bacterial bioluminescence (4/8 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.29

Use Curated BLAST to search for 1.16.1.3 or 1.5.1.29 or 1.5.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AW47 at UniProt or InterPro

Protein Sequence (233 amino acids)

>BWI76_RS01350 NAD(P)H-flavin reductase (Klebsiella michiganensis M5al)
MTTLSCKVTSVEAITDTVYRVRLVPEAAFAFRAGQYLMVVMDERDKRPFSMASTPSEQEF
IELHIGASELNLYAMAVMDRILKEREIEVDMPHGEAWLRDDEERPLILIAGGTGFSYVRS
ILLTALARNPNRDIAIYWGGREAKHLYDLAELEALSLQHPGLRVEPVVEQPEAEWRGRTG
TVLTAVLQDYGTLGEHDIYIAGRFEMAKIARDLFCNERGAREDRLFGDAFAFI