Protein Info for BWI76_RS01065 in Klebsiella michiganensis M5al

Annotation: ATP-dependent RNA helicase RhlB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 PF00270: DEAD" amino acids 33 to 206 (174 residues), 145 bits, see alignment E=2.9e-46 PF04851: ResIII" amino acids 52 to 179 (128 residues), 23 bits, see alignment E=9.8e-09 PF00271: Helicase_C" amino acids 242 to 351 (110 residues), 106.3 bits, see alignment E=1.6e-34

Best Hits

Swiss-Prot: 98% identical to RHLB_KLEP7: ATP-dependent RNA helicase RhlB (rhlB) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K03732, ATP-dependent RNA helicase RhlB [EC: 3.6.4.13] (inferred from 97% identity to kpu:KP1_0143)

MetaCyc: 96% identical to ATP-dependent RNA helicase RhlB (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"ATP-dependent RNA helicase RhlB" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVX7 at UniProt or InterPro

Protein Sequence (421 amino acids)

>BWI76_RS01065 ATP-dependent RNA helicase RhlB (Klebsiella michiganensis M5al)
MSKTHLTEQKFSDFALHPAVIEALEKKGFHNCTPIQALALPLTLEGRDVAGQAQTGTGKT
MAFLTSTFHYLLTHPAIADRQVNQPRALIMAPTRELAVQIHADAEPLAQSTGLKLGLAYG
GDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQVVVLDEADRMYDLGFIKDIR
WLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEYVEVEPEQKTGHRIKEELFYPSN
EEKMRLLQTLLEEEWPDRAIIFANTKHRCEDIWGHLAADGHRVGLLTGDVAQKKRLRILD
EFTRGDIDILVATDVAARGLHIPAVTHVFNYDLPDDCEDYVHRIGRTGRAGASGHSISLA
CEEYALNLPAIETYIGHSIPVSKYNSDALMTELPRPLRLTRPRPGNGPRRNGPPRNRRRS
G