Protein Info for BWI76_RS01060 in Klebsiella michiganensis M5al

Annotation: guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 499 PF02541: Ppx-GppA" amino acids 20 to 300 (281 residues), 345 bits, see alignment E=3.8e-107 PF21447: Ppx-GppA_III" amino acids 307 to 479 (173 residues), 134.3 bits, see alignment E=4.2e-43

Best Hits

Swiss-Prot: 89% identical to GPPA_KLEP3: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (gppA) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: None (inferred from 89% identity to eae:EAE_07775)

MetaCyc: 86% identical to guanosine-5'-triphosphate,3'-diphosphate phosphatase (Escherichia coli K-12 substr. MG1655)
Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.40]

Predicted SEED Role

"Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (EC 3.6.1.40)" in subsystem Phosphate metabolism or Stringent Response, (p)ppGpp metabolism (EC 3.6.1.40)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVT7 at UniProt or InterPro

Protein Sequence (499 amino acids)

>BWI76_RS01060 guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (Klebsiella michiganensis M5al)
MSSTSLYAAIDLGSNSFHMLVVREVAGSIQTLSRIKRKVRLAAGLNGDNTLSAEAMERGW
QCLRLFAERLQDIPPTQIRVVATATLRLAVNAGEFLEKAREILGCAVQVISGEEEARLIY
QGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSLSMGCVTWLERYFADRSLTKEN
FDQAETAAREVLRPVADVLRYHGWKVCVGASGTVQALQEIMMAQGMDERITLAKLQQLKQ
RAIQCGRLEELEIEGLTLERALVFPSGLAILMAIFSELNIQCMTLAGGALREGLVYGMLH
LSIDQDIRSRTLRNIQRRFLVDTEQAQRVAQLAAHFVHQVENSWEIEPLCFDLLQSACQL
HEIGLSVEYKQAPLHAAWLVRNLDLPGFTPAQKKLLATLLLNQTSTVDLSSLHQQNAVPP
RVAEHMCRLLRLAILFASRRRDDLLPMITLAAVDENLTLTLPHGWMENHPLGREMVEQEC
QWQSYVHWPLLLNEDGAAK