Protein Info for BWI76_RS00790 in Klebsiella michiganensis M5al

Annotation: bifunctional aspartokinase II/homoserine dehydrogenase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 12 to 284 (273 residues), 145.1 bits, E=4.4e-46
• TIGR00657: aspartate kinase
  • amino acids 15 to 453 (439 residues), 215.7 bits, E=6.6e-68
• PF03447: NAD_binding_3
  • amino acids 465 to 600 (136 residues), 78.2 bits, E=1.3e-25
• PF00742: Homoserine_dh
  • amino acids 608 to 803 (196 residues), 144.9 bits, E=3.4e-46

Best Hits

Swiss-Prot: 92% identical to AK2H_ECOLI: Bifunctional aspartokinase/homoserine dehydrogenase 2 (metL) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 96% identity to eae:EAE_07600)

MetaCyc: 92% identical to fused aspartate kinase/homoserine dehydrogenase 2 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• norspermidine biosynthesis (4/6 steps found)
• L-lysine biosynthesis II (6/9 steps found)
• ectoine biosynthesis (3/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• superpathway of polyamine biosynthesis III (4/8 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

See A0A285AVS2 at UniProt or InterPro

Protein Sequence (810 amino acids)

>BWI76_RS00790 bifunctional aspartokinase II/homoserine dehydrogenase II (Klebsiella michiganensis M5al)
MSVIAQAGAKGRQLHKFGGSSLADAKCYLRVAGIMTEYSQAGDMMVVSAAGSTTNQLISW
LKLSQTDRLSAHQVQQSLRRYQMDLISGLLSPDAADTLIATFIQDLERLAGLLDGGVTDA
VYAEVVGHGEVWSARLMAAVLNHLGVEAAWLDARSFLRAERSPQPQVDEGLSYPLLQQLM
AQHPNKRLVVTGFISRNNAGETVLLGRNGSDYSATQIGALAGASRVTIWSDVAGVYSADP
RKVKDACLLPLLRLDEASELARLAAPVLHARTLQPVSGSDIDLQLRCSYTPDQGSTRIER
VLASGTGARIVTSHDDVCLIEFQVPGGQDFKLAHKELDAILKRAQLRPLAVGVHADRKLL
QFCYTSEVADSALKLLDEAGLPGELRLRQKLALVAMVGAGVTRNPLHCHRFWQQLKGQPV
EFTWQSEEGISLVAVLRAGPTESLIQGLHQSLFRAEKRIGLVLFGKGNIGSRWLELFARE
QTTLSARTGFEFILAGVVDSRRSLLNYEGLDASRALAFFNDEAIEQDEESLFLWMRAHPY
DDLVVLDVTASAQLADQYLDFASHGFHVISANKLAGASSSSKYRQIHDAFEKTGRHWLYN
ATVGAGLPVNHTVRDLIDSGDTILALSGIFSGTLSWLFLQFDGTVPFTDLVDQAWQQGLT
EPDPRVDLSGKDVMRKLVILAREAGYDIEPDQVRVESLVPAHCEEGSVDHFFENGEALND
QMLQRLEAAREMGLVLRYVARFDANGKARVGVEAVREEHPLAALLPCDNVFAIESRWYRD
NPLVIRGPGAGRDVTAGAIQSDINRLAQLL