Protein Info for BWI76_RS00600 in Klebsiella michiganensis M5al

Annotation: LacI family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 331 PF00356: LacI" amino acids 3 to 47 (45 residues), 45.5 bits, see alignment 1e-15 PF00532: Peripla_BP_1" amino acids 60 to 309 (250 residues), 180.6 bits, see alignment E=9.7e-57 PF13377: Peripla_BP_3" amino acids 171 to 330 (160 residues), 56.9 bits, see alignment E=5.8e-19

Best Hits

Swiss-Prot: 64% identical to RAFR_ECOLX: HTH-type transcriptional regulator RafR (rafR) from Escherichia coli

KEGG orthology group: K02529, LacI family transcriptional regulator (inferred from 91% identity to kpe:KPK_5479)

Predicted SEED Role

"Putative ThuR, regulatory protein for trehalosemaltose transport"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVN6 at UniProt or InterPro

Protein Sequence (331 amino acids)

>BWI76_RS00600 LacI family transcriptional regulator (Klebsiella michiganensis M5al)
MSLKAIAKELGISVTTVSRALNGYDDVSQETRARVEAEAQRRGYRPNTFARRLKMGKIDA
VGLVFPVRPAPLNNNVFLEMVGEISHELARYEIDLLLIADDEQADKHGYMRMVQSRRVDA
LIVAHTLDDDPRLIQLLSAGFPFLALGRSRLPQPYAWFDFDNQAGTYRATRHLISQGHRR
IALLSENNNQAFITQRRNGYLQALREAGLADTWLRSVPPTRRGGFQATMELLRLPEPPTA
IVTDCNTHGDGAAMALAQLGRLTGDNRVALVVYDGLPQDSIIETPVGAVIQSTRQGVGRQ
IASMVRRLIAGDDLASLQVLWQPEFISGETA