Protein Info for BWI76_RS00510 in Klebsiella michiganensis M5al
Annotation: glucuronyl hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 46% identical to UGL_BACGL: Unsaturated glucuronyl hydrolase (ugl) from Bacillus sp. (strain GL1)
KEGG orthology group: None (inferred from 86% identity to cro:ROD_38581)MetaCyc: 46% identical to unsaturated beta-glucuronyl hydrolase (Bacillus sp. GL1)
RXN-14021 [EC: 3.2.1.179, 3.2.1.180]; 3.2.1.179 [EC: 3.2.1.179, 3.2.1.180]; 3.2.1.179 [EC: 3.2.1.179, 3.2.1.180]
Predicted SEED Role
"Unsaturated glucuronyl hydrolase (EC 3.2.1.-)" (EC 3.2.1.-)
MetaCyc Pathways
- gellan degradation (1/2 steps found)
- hyaluronan degradation (1/2 steps found)
- dermatan sulfate degradation I (bacterial) (1/4 steps found)
- chondroitin sulfate degradation I (bacterial) (2/8 steps found)
KEGG Metabolic Maps
- Ascorbate and aldarate metabolism
- Glycosaminoglycan degradation
- Nucleotide sugars metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.-
Use Curated BLAST to search for 3.2.1.- or 3.2.1.179 or 3.2.1.180
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A285AVI9 at UniProt or InterPro
Protein Sequence (392 amino acids)
>BWI76_RS00510 glucuronyl hydrolase (Klebsiella michiganensis M5al) MTIALEALDSRYFLPQPGLDRAWLDGAIGDVLIRLDAMLPRFTATFPAASASHGRYESVE RVDWTEGFWTGMLWLAWEVTGDEKYRAVAESLLDSFEERLDKEIKVDTHDLGFLYLLSCV NAWKLTGNLRARELALRAAELLYRRFNATAGVIQAWGDLNDPARQGRMIIDCNLNVPLLF WAANETGNPRWREAASRHLAQAARYLVREDASTFHTFYMDVHNGKPLRGDTHQGFSDSSC WSRGQAWGIYGFALGYTHTGDAWQPELSRRLTHYFLNRLPEDFVCYWDLIFTAEDNQYRD TSAAAIAACGLAELLKLLPLTDPLRPAYGNAIELMMRNLRERYFAHAQDGLLREGVYNFG RNMGINEPNLWGDYFYFEALVRLSRVWTPYFC