Protein Info for BWI76_RS00465 in Klebsiella michiganensis M5al

Annotation: formate dehydrogenase-N subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 1 to 803 (803 residues), 1248.9 bits, see alignment E=0 PF00384: Molybdopterin" amino acids 2 to 377 (376 residues), 61.1 bits, see alignment E=1e-20 PF01568: Molydop_binding" amino acids 682 to 797 (116 residues), 61.9 bits, see alignment E=5.8e-21

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 66% identity to alv:Alvin_2452)

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVJ0 at UniProt or InterPro

Protein Sequence (804 amino acids)

>BWI76_RS00465 formate dehydrogenase-N subunit alpha (Klebsiella michiganensis M5al)
MTNHWVDIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADFY
TPIRSGTDITFLSGVILYLLNNEKINREYTEAYTNASLIVREDYSFEDGLFSGYDAEARK
YDKTSWNYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPEVVENICGTPKADFLKVCE
YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQ
GLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLKGQVNYWGNYPKFFVSMMKAF
FGDKATAENSWGFDWLPKWDKGYDVLQYFEMMNQGKVNGYICQGFNPVASFPNKNKVVAS
LSKLKFLVTIDPLNTETSTFWQNHGESNDVDPTKIQTEVFRLPSTCFAEENGSIVNSGRW
LQWHWKGADAPGIATTDGEILAGIFLRLRKMYAEQGGPAPEPVLNMTWNYATPHEPASEE
VAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSSGCWIFAGSWTPDGNQMA
RRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPAGKPWDPKRQLLKWDGAKWGGVDIA
DYSAAAPDSDVGPFIMQPEGMGRLFAIDKMAEGPFPEHYEPFETPLGTNPLHPNVVSNPA
ARVFKGDLEQMGKADKFPYVGTTYRLTEHFHYWTKHALLNAIAQPEQFVEIGEKLANKLG
IAHGDTVKVSSNRGYIKAKAVVTKRIRTLKADGKDIDTIGIPIHWGYEGVAKKGFIANTL
TPFVGDANTQTPEFKAFLVNVEKA