Protein Info for BWI76_RS00415 in Klebsiella michiganensis M5al

Annotation: glucose-1-phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 199 PF00702: Hydrolase" amino acids 2 to 178 (177 residues), 55.8 bits, see alignment E=1.3e-18 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 65 to 184 (120 residues), 64.9 bits, see alignment E=4.9e-22 PF13419: HAD_2" amino acids 70 to 184 (115 residues), 42.5 bits, see alignment E=1.2e-14 PF13242: Hydrolase_like" amino acids 139 to 186 (48 residues), 26.4 bits, see alignment E=7.7e-10

Best Hits

Swiss-Prot: 86% identical to YIHX_SHIFL: Alpha-D-glucose-1-phosphate phosphatase YihX (yihX) from Shigella flexneri

KEGG orthology group: K07025, putative hydrolase of the HAD superfamily (inferred from 90% identity to kpn:KPN_04180)

MetaCyc: 86% identical to alpha-D-glucose-1-phosphate phosphatase YihX (Escherichia coli K-12 substr. MG1655)
Glucose-1-phosphatase. [EC: 3.1.3.10]

Predicted SEED Role

"Alpha-D-glucose-1-phosphatase"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.10

Use Curated BLAST to search for 3.1.3.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVI2 at UniProt or InterPro

Protein Sequence (199 amino acids)

>BWI76_RS00415 glucose-1-phosphatase (Klebsiella michiganensis M5al)
MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLQQHFTMGEAFHQHERGEISDEDFAAA
MCHEMNMSLSYEQFSHGWQAVFVSLRPEVIAIMQKLREQGHRVVVLSNTNRLHTTFWPDE
YPEIRAAADHIYLSQEMGMRKPEARIYQSVLEAEGFSADDTVFFDDNADNIAGANQLGIT
SILVTGKETIPNYFAKQLC