Protein Info for BWI76_RS00270 in Klebsiella michiganensis M5al

Updated annotation (from data): D-ribose pyranase (EC 5.4.99.62)
Rationale: Specifically important for utilizing D-Ribose. Automated validation from mutant phenotype: the predicted function (RXN0-5304) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: D-ribose pyranase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF05025: RbsD_FucU
  • amino acids 1 to 138 (138 residues), 155.2 bits, E=7.2e-50

Best Hits

Swiss-Prot: 92% identical to RBSD_KLEP3: D-ribose pyranase (rbsD) from Klebsiella pneumoniae (strain 342)

KEGG orthology group: K06726, D-ribose pyranase [EC: 5.-.-.-] (inferred from 91% identity to kpn:KPN_04153)

MetaCyc: 86% identical to D-ribose pyranase (Escherichia coli K-12 substr. MG1655)
RXN0-5304 [EC: 5.4.99.62]

Predicted SEED Role

"Ribose ABC transport system, high affinity permease RbsD (TC 3.A.1.2.1)" in subsystem D-ribose utilization (TC 3.A.1.2.1)

MetaCyc Pathways

• ribose phosphorylation (2/2 steps found)

KEGG Metabolic Maps

• Biosynthesis of steroids
• Carotenoid biosynthesis - General
• Lipopolysaccharide biosynthesis
• Pentose and glucuronate interconversions

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-

Use Curated BLAST to search for 5.-.-.- or 5.4.99.62

Sequence Analysis Tools

See A0A285AVD9 at UniProt or InterPro

Protein Sequence (139 amino acids)

>BWI76_RS00270 D-ribose pyranase (EC 5.4.99.62) (Klebsiella michiganensis M5al)
MKKGTVLNADISSVISRLGHTDTLVVCDAGLPVPRSSTRIDMALTHGVPSFMQVLEVVTS
EMQVEVAILAEEIKTLNPQLHATLLNHLEQLQQHQGNTIEIRYTSHEQFKKETADSQAVI
RSGECSPYANVILCAGVTF