Protein Info for BWI76_RS00175 in Klebsiella michiganensis M5al

Annotation: pectate disaccharide-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF13229: Beta_helix" amino acids 451 to 626 (176 residues), 38.3 bits, see alignment E=5.5e-14

Best Hits

Swiss-Prot: 62% identical to PLYX_DICCH: Pectate disaccharide-lyase (pelX) from Dickeya chrysanthemi

KEGG orthology group: K01731, pectate disaccharide-lyase [EC: 4.2.2.9] (inferred from 74% identity to ent:Ent638_4134)

Predicted SEED Role

"Exopolygalacturonate lyase (EC 4.2.2.9)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 4.2.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.2.9

Use Curated BLAST to search for 4.2.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVC7 at UniProt or InterPro

Protein Sequence (723 amino acids)

>BWI76_RS00175 pectate disaccharide-lyase (Klebsiella michiganensis M5al)
MKNKIPLALTLCSTMIALPLQANEGHQWRAIAFGQSTDSNFSSNVLPEKIGVNDVTIAGK
KLTPQDSADLSQPITIESRGGKIANSHDGLTFFYTELPTNKNFILQATVTVNQFGPENGA
RPAAQEGAGLLVRDVIGNPRQQPLKVGYEEFPAASNMVMNAIMTQDKKDGSHIKLQAISR
EGITQPWGNAGAAIKRQSYKEKIDIQQTPTFQLKLERTNDGFITSWAATGSNEWVSQRVP
HADLIAQQDKEHYYVGFFASRNAKITVSNASLTTSAANTVPSAPYVAKSWPPVMQIASGT
KSQSKEYLLQARTNSDGRITVRQDEVVIGQDKAVKAGEMYTQPAVLKDKSTFEISFTPAT
GANTLTQTLTVEQSANVTGNTLYAAPDGLSQAKGTTDSPLDLATAVDLVPPGGQIVLAAG
DYPQTTIPVSASGLKDKVKTLKADGKAVIHGLLLDASYWHINGVDITDKSLRVQGSHNLI
ENVTAYRNDDTGIQISSPADVGRPLWASHNRVVNSESFNNEDPGKINADGFAVKMRVGEG
NRLEGCYSHDNIDDGFDLFNKIEDGANGVVVIENSIARNNTSNGFKLGGEGQPVAHEVRN
SIAIGNHLDGFTDNFNPGKLVVVNNVAVDNQRFNYLFRPSPYGAPETQGTFSDNLSLRSQ
PGKYDDAVIGNIDDSNYFIHGGRSINAQGKSIHSADYQTLTLPDPLTREADGSFNTGNFL
SRN