Protein Info for BWI76_RS00170 in Klebsiella michiganensis M5al

Annotation: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 6 to 47 (42 residues), 28.3 bits, 3.6e-10
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 6 to 455 (450 residues), 682.2 bits, E=1.7e-209
• PF00483: NTP_transferase
  • amino acids 8 to 213 (206 residues), 48.8 bits, E=1.8e-16
• PF12804: NTP_transf_3
  • amino acids 8 to 121 (114 residues), 73.7 bits, E=5e-24
• PF00132: Hexapep
  • amino acids 268 to 300 (33 residues), 31.5 bits, see alignment (E = 2.5e-11)
  • amino acids 395 to 429 (35 residues), 37.3 bits, 3.6e-13
• PF14602: Hexapep_2
  • amino acids 395 to 429 (35 residues), 32.8 bits, 1.1e-11

Best Hits

Swiss-Prot: 92% identical to GLMU_KLEP7: Bifunctional protein GlmU (glmU) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: None (inferred from 93% identity to eae:EAE_07155)

MetaCyc: 88% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See A0A285AVC1 at UniProt or InterPro

Protein Sequence (456 amino acids)

>BWI76_RS00170 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (Klebsiella michiganensis M5al)
MSNSPMSVVILAAGKGTRMYSDLPKVLHSLAGKPMVQHVIDAAKELGASAVHLVYGHGGD
LLRQTLREDNLNWVLQAEQLGTGHAMQQAAPFFSDDEDILMLYGDVPLISVETLQRLRDA
KPQGGIGLLTVKLDDPTGYGRITRDNGAVTGIVEHKDASEEQRRIQEINTGILVANGADL
KRWLAKLTNNNVQGEYYITDIIAMAYQEGREITAVHPRRLSEVEGVNNRLQLSRLERVWQ
SEQAEKLLLAGVMLRDPARFDLRGVLKHGRDVEIDTNVILEGDVVLGDRVKIGAGCVIKN
STIGDDCEISPYTVVEDAHLEAACTIGPFARLRPGAELLEGAHVGNFVEIKKARLGKGTK
AGHLSYLGDAEIGDNVNIGAGTITCNYDGANKHKTVIGDDVFVGSDSQLVAPVTVGNGVT
IAAGTTVTRDIADNELVLSRVPQVHKQGWQRPVKKK