Protein Info for BWI76_RS00165 in Klebsiella michiganensis M5al

Annotation: glutamine--fructose-6-phosphate aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 609 (608 residues), 888.9 bits, E=8.7e-272
• PF13230: GATase_4
  • amino acids 54 to 129 (76 residues), 24.1 bits, E=3.1e-09
• PF13522: GATase_6
  • amino acids 65 to 182 (118 residues), 68.8 bits, E=9.4e-23
• PF13537: GATase_7
  • amino acids 81 to 195 (115 residues), 51.6 bits, E=1.8e-17
• PF01380: SIS
  • amino acids 290 to 418 (129 residues), 117.1 bits, E=1e-37
  • amino acids 462 to 591 (130 residues), 99.9 bits, E=2e-32

Best Hits

Swiss-Prot: 93% identical to GLMS_SHIFL: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Shigella flexneri

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 93% identity to eco:b3729)

MetaCyc: 93% identical to L-glutamine--D-fructose-6-phosphate aminotransferase (Escherichia coli K-12 substr. MG1655)
Glutamine--fructose-6-phosphate transaminase (isomerizing). [EC: 2.6.1.16]

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (11/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Sequence Analysis Tools

See A0A285AVB1 at UniProt or InterPro

Protein Sequence (609 amino acids)

>BWI76_RS00165 glutamine--fructose-6-phosphate aminotransferase (Klebsiella michiganensis M5al)
MCGIVGAVAQRDIAEILLEGLRRLEYRGYDSAGLAVVDNEGHMNRVRRLGKVQMLAQALE
EHPLHGGTGIAHTRWATHGEPSEGNAHPHVSDHIVVVHNGIIENHEPLRELLQSRGYVFV
SETDTEVIAHLVHWELEQGGTLREAVLRTIPQLRGAYGTVIMDTRDPGTLLAARSGSPLV
IGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEVTRRSVTIFDKSGAEVQRPDIESNLQ
YDAGDKGIYRHYMQKEIYEQPNAIKNTLSGRISHGEVDLSELGPQANELLSQVEHIQIIA
CGTSYNSGMVSRYWFESLAGVPCDVEIASEFRYRKSAVRRNSLMITLSQSGETADTLAGL
RLSKELGYLGSLAICNVPGSSLVRESDLAMMTNAGTEIGVASTKAFTTQLTVLLMLVAKL
ARLKGQDAAIEHEIVHGLQALPSRIEQVLSQDKRIEALAENFSDKHHALFLGRGDQYPIA
LEGALKLKEISYIHAEAYAAGELKHGPLALIDAEMPVVVVAPNNELLEKLKSNIEEVRAR
GGQLYVFADGDAGFTSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPR
NLAKSVTVE