Protein Info for BWI76_RS00070 in Klebsiella michiganensis M5al

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details amino acids 55 to 76 (22 residues), see Phobius details amino acids 86 to 106 (21 residues), see Phobius details amino acids 112 to 134 (23 residues), see Phobius details amino acids 146 to 165 (20 residues), see Phobius details amino acids 171 to 190 (20 residues), see Phobius details amino acids 224 to 243 (20 residues), see Phobius details amino acids 249 to 267 (19 residues), see Phobius details amino acids 274 to 294 (21 residues), see Phobius details amino acids 313 to 335 (23 residues), see Phobius details amino acids 344 to 368 (25 residues), see Phobius details amino acids 372 to 393 (22 residues), see Phobius details amino acids 404 to 425 (22 residues), see Phobius details amino acids 432 to 452 (21 residues), see Phobius details TIGR00891: MFS transporter, sialate:H+ symporter (SHS) family" amino acids 9 to 441 (433 residues), 586.8 bits, see alignment E=1.2e-180 PF00083: Sugar_tr" amino acids 24 to 202 (179 residues), 79.6 bits, see alignment E=2.4e-26 PF07690: MFS_1" amino acids 26 to 241 (216 residues), 100.3 bits, see alignment E=1.1e-32 amino acids 280 to 451 (172 residues), 36.1 bits, see alignment E=3.7e-13

Best Hits

Swiss-Prot: 88% identical to NANT_SALHS: Sialic acid transporter NanT (nanT) from Salmonella heidelberg (strain SL476)

KEGG orthology group: K03290, MFS transporter, SHS family, sialic acid transporter (inferred from 87% identity to cro:ROD_45981)

MetaCyc: 86% identical to N-acetylneuraminate:H+ symporter (Escherichia coli K-12 substr. MG1655)
RXN-15314

Predicted SEED Role

"Sialic acid transporter (permease) NanT" in subsystem Sialic Acid Metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A285AVA7 at UniProt or InterPro

Protein Sequence (496 amino acids)

>BWI76_RS00070 MFS transporter (Klebsiella michiganensis M5al)
MSEIISTKPWYKHLNKSQWRAFSAAWLGYLLDGFDFVLIALVLTEVQSEFQLTTVQAASI
ISAAFISRWFGGLLLGAMGDRYGRRLAMVSSIILFSVGTLACGFAPGYTSMFIARMVIGM
GMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVAAQVYSLVVPLWGWRALFFLG
ILPIIFALWLRKNIPETEDWKERQTKKAPVRTMVDILYRGKYRAMNIATTLIATAALWFC
FAGNQHNPLVVIALALVCTVIFISFMVQSSGKRWPTGVMLMVVVLFAFLYSWPIQALLPT
YLKTELAYDPAAVAKVLFFSGFGAAVGCIVGGFLGDWLGTRKAYVYSLLASQVLIIPVFA
IGGASLWGLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQRAAGLGFTYNVGALGGAAAPV
LGALVAQHMHLGTALGSLSFGLTFVVILLIGLDMPSRVQRWIRPEALRTHDAIDGKPFSG
AKVRHDKANAPLCSKH