Protein Info for BPHYT_RS36320 in Burkholderia phytofirmans PsJN

Annotation: type IV secretion system family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 234 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF07996: T4SS" amino acids 23 to 217 (195 residues), 127 bits, see alignment E=6.4e-41

Best Hits

KEGG orthology group: K03200, type IV secretion system protein VirB5 (inferred from 100% identity to bpy:Bphyt_7348)

Predicted SEED Role

"Minor pilin of type IV secretion complex (VirB5)" in subsystem Type 4 secretion and conjugative transfer

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TH84 at UniProt or InterPro

Protein Sequence (234 amino acids)

>BPHYT_RS36320 type IV secretion system family protein (Burkholderia phytofirmans PsJN)
MMIRKLVVSTFAAFSFSLANAQGVPVYDNAANIALLQQVVSAGQQLAQLQQQYQQLQQTF
NALNGLRNISSLLSNPLLSQMLPPDLASTINSLRSGNVTGSLAGISGNLQQLAHQNAKID
CQNQWGPNSPSYYSCVNKWQQASFSSYVGQQGYDQSAQNIANLQQFLSAIQTSPDPKSLQ
DLQARISLEQVKQQNEAIKLKGIEMMQQAQEKMDHQNAVQSTQQMMLTGQGIRF