Protein Info for BPHYT_RS35670 in Burkholderia phytofirmans PsJN

Annotation: bicyclomycin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 410 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF01547: SBP_bac_1" amino acids 45 to 324 (280 residues), 185.9 bits, see alignment E=2.8e-58 PF13416: SBP_bac_8" amino acids 49 to 350 (302 residues), 143.2 bits, see alignment E=2.1e-45

Best Hits

Swiss-Prot: 58% identical to Y3748_BRUSU: Putative binding protein BRA0748/BS1330_II0741 (BRA0748) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K02027, multiple sugar transport system substrate-binding protein (inferred from 100% identity to bpy:Bphyt_7208)

Predicted SEED Role

"Inositol transport system sugar-binding protein" in subsystem Inositol catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TAJ1 at UniProt or InterPro

Protein Sequence (410 amino acids)

>BPHYT_RS35670 bicyclomycin resistance protein (Burkholderia phytofirmans PsJN)
MSLHARASLALGKVAVALAFAGIAVAAHADTVRVTVAHYSDATAPYFEKMARNFEKANPG
TTIKIEDVNWDTLQQKLQTDISGNANADLAIVGTRWLLDFVKDDVAEPLDGYMDANFKSR
FIGPFLAPGEINGKVYGLPIAASARGLYYNKDLLAKVGYPDGPKTWNDVIEASKKLKAQG
IAGFGLQGKEIETDVYYYYALWTNGGDVVGKDGKAAFNSPAGIKAATLYKTMIDQGLTQP
GVTGYSREDVQNLFKQGRVAMMISAPFLAKQIKKEAPNLKYGIDPIPMGTTHATYAVTDS
IVMFKNSKVKKSAWKFLDYLFTKEPRVEFTTTEGFLPTTKAEATDPAFNDPDTKAFVALL
PTAHFAPTVTGWEDTAKAVTDAMQSIYLGKAKPADALNAAAAQANKSLGK