Protein Info for BPHYT_RS35025 in Burkholderia phytofirmans PsJN

Annotation: sarcosine oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF01266: DAO" amino acids 2 to 348 (347 residues), 153.8 bits, see alignment E=3.2e-48 PF00890: FAD_binding_2" amino acids 3 to 33 (31 residues), 21.1 bits, see alignment (E = 5.9e-08) PF13450: NAD_binding_8" amino acids 5 to 38 (34 residues), 35.6 bits, see alignment 3.2e-12 PF01593: Amino_oxidase" amino acids 132 to 203 (72 residues), 25 bits, see alignment E=4e-09

Best Hits

KEGG orthology group: K00301, sarcosine oxidase [EC: 1.5.3.1] (inferred from 100% identity to bpy:Bphyt_7077)

Predicted SEED Role

"Monomeric sarcosine oxidase (EC 1.5.3.1)" (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2TA63 at UniProt or InterPro

Protein Sequence (367 amino acids)

>BPHYT_RS35025 sarcosine oxidase (Burkholderia phytofirmans PsJN)
MRVTIVGAGIAGLSTAWSLSKQGHDITLIEQGSIPNPLAASGDRHRMIRRAYGDADGYAR
NIEEAFDSWDLLWNDLGVSHYANCGVLGISQFAGDGAEQFRTGLDRMGFEYERLEPLDAA
ARYPFLDPATFRYAYLDHDGGALFSERIARDMKAWLKMRGADIRMHTKVHAVDPNAASVQ
LENDEIVRADRLVVTAGAWTAQMFPALAENLMTYRNIVAYLEPPADLEDAWATAPAIVDI
GGASDGYVLPPLDGIGLKFGAGVNKARTPDPEANRAAAPGEGDRLRRLFSPPFSRIDEYT
VSEVKTCAYTFTADNRFFSKRIGNTLVVSACSGHGYKFGAAVGRRIAEALETNDDATLAR
WLRAETV