Protein Info for BPHYT_RS34230 in Burkholderia phytofirmans PsJN
Updated annotation (from data): L-fuconate dehydratase; L-rhamnonate dehydratase (EC 4.2.1.68; EC 4.2.1.90)
Rationale: Specifically important for: L-Rhamnose monohydrate; L-Fucose. annotated by SEED for fuconate activity only
Original annotation: hydrolase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01685, altronate hydrolase [EC: 4.2.1.7] (inferred from 100% identity to bpy:Bphyt_6916)Predicted SEED Role
"L-fuconate dehydratase (EC 4.2.1.68), type 2" (EC 4.2.1.68)
MetaCyc Pathways
- L-rhamnose degradation III (6/7 steps found)
- D-galacturonate degradation I (4/5 steps found)
- superpathway of hexuronide and hexuronate degradation (7/10 steps found)
- L-fucose degradation III (5/8 steps found)
- L-rhamnose degradation II (5/8 steps found)
- L-fucose degradation II (2/5 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (20/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.68, 4.2.1.7, 4.2.1.90
Use Curated BLAST to search for 4.2.1.68 or 4.2.1.7 or 4.2.1.90
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See B2T9V6 at UniProt or InterPro
Protein Sequence (431 amino acids)
>BPHYT_RS34230 L-fuconate dehydratase; L-rhamnonate dehydratase (EC 4.2.1.68; EC 4.2.1.90) (Burkholderia phytofirmans PsJN) MPVAAQQPTLEGYLRGDGRKGIRNVVAVAYLVECAHHVAREIVTQFREPLDAFDDPSAER EPPVHLIGFPGCYPNGYAEKMLERLTTHPNVGAVLFVSLGCESMNKHYLVDVVRASGRPV EVLTIQEKGGTRSTIQYGVDWIRGAREQLAAQQKVPMALSELVIGTICGGSDGTSGITAN PAVGRAFDHLIDAGATCIFEETGELVGCEFHMKTRAARPALGDEIVACVAKAARYYSILG HGSFAVGNADGGLTTQEEKSLGAYAKSGASPIVGIIKPGDIPPTGGLYLLDVVPDGEPRF GFPNISDNAEIGELIACGAHVILFTTGRGSVVGSAISPVIKVCANPATYRNLSGDMDVDA GRILEGRGTLDEVGREVFEQTVAVSRGAASKSETLGHQEFILTYKTFEPVGPACLPSSAA AQHRVVAIEPH