Protein Info for BPHYT_RS34135 in Burkholderia phytofirmans PsJN

Annotation: glutathione S-transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13417: GST_N_3
  • amino acids 12 to 82 (71 residues), 34.6 bits, E=4e-12
• PF13409: GST_N_2
  • amino acids 13 to 78 (66 residues), 49.4 bits, E=1.1e-16
• PF02798: GST_N
  • amino acids 15 to 77 (63 residues), 28.6 bits, E=2.9e-10
• PF13410: GST_C_2
  • amino acids 128 to 201 (74 residues), 31.5 bits, E=3.1e-11

Best Hits

KEGG orthology group: K04097, glutathione S-transferase [EC: 2.5.1.18] (inferred from 100% identity to bpy:Bphyt_6895)

Predicted SEED Role

"Glutathione S-transferase (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)

MetaCyc Pathways

• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• pentachlorophenol degradation (5/10 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• gliotoxin biosynthesis (2/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18

Sequence Analysis Tools

See B2T9T5 at UniProt or InterPro

Protein Sequence (217 amino acids)

>BPHYT_RS34135 glutathione S-transferase (Burkholderia phytofirmans PsJN)
MANTTLTISSKNYSSWSLRGWLLTKFSGLPFEEIVMPIDDPAARAELLLLSPSILVPCLV
HDGIKVWDTLAIAEYLNELKPDAKLLPEDIRARAHCRSICGEMHSGFGSLRSALPMNLKA
HFPGFKVWARAQSDIDRIVTIWGDCLKQYGGPFLFGERTAADAMYAPVVTRFVTYDVKLD
PEIVEYGQRIMALPEMQEWIADAQQEVEEIDELDVEF