Protein Info for BPHYT_RS34125 in Burkholderia phytofirmans PsJN

Annotation: heavy metal translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 872 transmembrane" amino acids 199 to 218 (20 residues), see Phobius details amino acids 229 to 248 (20 residues), see Phobius details amino acids 260 to 282 (23 residues), see Phobius details amino acids 288 to 306 (19 residues), see Phobius details amino acids 446 to 465 (20 residues), see Phobius details amino acids 473 to 497 (25 residues), see Phobius details amino acids 818 to 836 (19 residues), see Phobius details amino acids 842 to 862 (21 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 22 to 83 (62 residues), 35.2 bits, see alignment 2.9e-12 amino acids 105 to 167 (63 residues), 35.1 bits, see alignment 3e-12 PF00403: HMA" amino acids 23 to 82 (60 residues), 61.5 bits, see alignment 1.6e-20 amino acids 106 to 166 (61 residues), 64.2 bits, see alignment 2.4e-21 TIGR01525: heavy metal translocating P-type ATPase" amino acids 262 to 860 (599 residues), 606.9 bits, see alignment E=1.1e-185 TIGR01494: HAD ATPase, P-type, family IC" amino acids 299 to 561 (263 residues), 155.7 bits, see alignment E=3.7e-49 amino acids 657 to 838 (182 residues), 126.9 bits, see alignment E=2e-40 PF00122: E1-E2_ATPase" amino acids 327 to 506 (180 residues), 185.4 bits, see alignment E=1.5e-58 PF00702: Hydrolase" amino acids 524 to 772 (249 residues), 106 bits, see alignment E=6.7e-34

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to bpy:Bphyt_6893)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9T3 at UniProt or InterPro

Protein Sequence (872 amino acids)

>BPHYT_RS34125 heavy metal translocating P-type ATPase (Burkholderia phytofirmans PsJN)
MTELANPQRPADAASPSARTAELDIGGMTCASCALRVEKALAKVPGVTRASVNLATERAR
IESDAGVDPETLANAVRKAGYDAMLSASTSTETPAPVAEAEHSTELAIGGMTCASCAMRV
EKALAKVPGVVGASVNLATETATVSLDGAAAAPDALIAAVRKAGYEATLIVPPDASAAVD
HADQTAPADRKRDQTRRELAAVLASAVLTLPLVAPMVGEWFGLHAMLSPWLQLALASIVQ
FVFGARFYRAAYRAVRAGAGNMDLLVALGTSAAYGISVYELATHPGDMMHLYFEASAVVI
TLVRFGKWLEARAKRQTTDAIRALNALRPDRARIRVGADERDVPLAQVRVGTVVIVRPGE
RVPVDGAVLEGRTHIDESLITGESLPVPKQVADAVTAGSINGEGAIAVTTTAIGAETTLA
RIIRLVESAQAEKAPIQRLVDRVSEIFVPAILAIAALTLVGWLMAGAGGETAILNAVAVL
VIACPCALGLATPAAIMAGTGVAARHGVLIKDAEALETAHRVTIVAFDKTGTLTLGQPSV
TAFEPIGGIGRDEALALAAAVQRNSDHPLARAVLKAHEVAVRNSGDGTVADTGGAVATDT
GIALASGQITPQHASAARAIAGRGVEADVDGRTLALGSTRWLKELGIDLPPELAARAHEL
EAAGNTVSWLMQRKPQAPAALALIAFGDTVKPTARVAVERLAQMGIKSVLVTGDNRGSAA
SVAKALGIGEFHAEVLPDDKARVIRDLKIRSAGIVAMAGDGINDAPALAAADIGIAMATG
TDVAMHAAGITLMRGDPALVADAIDISRRTWRKIQQNLFWAFVYNLIGIPLAALGLLNPM
LAGAAMAFSSVSVVTNALLLRTWRGEQGRSAR