Protein Info for BPHYT_RS34040 in Burkholderia phytofirmans PsJN

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF16697: Yop-YscD_cpl" amino acids 20 to 94 (75 residues), 34 bits, see alignment E=4.3e-12 PF00498: FHA" amino acids 22 to 86 (65 residues), 42 bits, see alignment E=1.6e-14 PF00437: T2SSE" amino acids 286 to 556 (271 residues), 222.3 bits, see alignment E=9.7e-70

Best Hits

KEGG orthology group: K02283, pilus assembly protein CpaF (inferred from 100% identity to bpy:Bphyt_6876)

Predicted SEED Role

"Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily" in subsystem Widespread colonization island

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9R6 at UniProt or InterPro

Protein Sequence (634 amino acids)

>BPHYT_RS34040 hypothetical protein (Burkholderia phytofirmans PsJN)
MLNLQIHPRNAPVTSLQVEQGCTIGKDASCDVIVKGMLIGKLQARIVRENNAYYIEDQGG
IAATLVNGSPITRYGPLTEADQIEIGVTTIKIVRAAATTQSAQSAHHAAQPSQVAAQAPI
SLSAAPSAQASGPAQFGSSFVSEAQAPSPTAAPYAQAMAPIAESIDRSVAMVRESAVAPS
KPAAEAPLRMHSAQVKKTAAESLPVAPINSPVGIELRKQAHMKVIAALDLRRLNVARMEE
DDLRKTVSAALDDILNHDASFRSPDIALETLKKSVFDEIIGLGPLEELLADPTVSEIMVN
CHDEIFVEQAGQLKRTPVIFTDDRAVIGAIERIVAPIGRRIDESSPMVDARLADGSRVNA
VIPPLALKGPSITIRKFSKRKLVGQDLLNFGTMSPEMLEFLHTAVEQGANIIISGGTGSG
KTTLLNVLSSYIPDSERIVTVEDAAELQLSQPNLVSLEARPANMEGKGAIHIRDLVKNCL
RMRPDRIVIGECRGGEALDMLQAMNTGHDGSLTTAHANTPRDCIARLEVMTLMAGLDLPV
QAIREQICSAVDIIVQQSRFSCGSRRVTHVTEVSGMESGVITLQDVFVFKEEGFGENGKI
VGKFVPTAYIPDFYQELIRRRIPVNTDIFTRAAG