Protein Info for BPHYT_RS33785 in Burkholderia phytofirmans PsJN

Annotation: glycogen debranching protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 739 transmembrane" amino acids 511 to 529 (19 residues), see Phobius details TIGR02100: glycogen debranching enzyme GlgX" amino acids 12 to 695 (684 residues), 1024.8 bits, see alignment E=0 PF02922: CBM_48" amino acids 16 to 104 (89 residues), 64.8 bits, see alignment E=7.1e-22 PF00128: Alpha-amylase" amino acids 200 to 301 (102 residues), 30.2 bits, see alignment E=3.7e-11

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to bpy:Bphyt_6824)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See B2T9L5 at UniProt or InterPro

Protein Sequence (739 amino acids)

>BPHYT_RS33785 glycogen debranching protein (Burkholderia phytofirmans PsJN)
MSHAMPDRLLPGSPYPLGASWDGLGVNFAVFSANAQKIELCLFDPTGRKEIRRYTLPECT
DEVWHGYLPNAHPGTAYGFRAHGPYQPQHGHRFNPHKLLLDPYARKLVGQFRWSDALFGY
RVHSNRADLSIDRRDSAPAMPKCVVIDEAFDWSHDKRPNVPWGETIVYETHVRGASMLRA
DLRQHERGTFAALSSPEFIEHLLKLGVTAVELLPVHAFLNDRFLVERGLRNYWGYNTAAF
FAPEPSYLSTHRLDEMRIAVRQLHAAGIEVILDVVYNHTCEGNEMGPTVSWRGLDNASYY
RLIPGDERHHINDTGCGNTVNLPHPRVLQMVMDSLRYWSTAFNIDGFRFDLGVTLGREQH
GFDPGSGFFDALRQDPILSQRKLISEPWDIGPGGYQLGNHPPGFSEWNDRFRDTVRRFWR
GDAGLRPDLAARLTGSADLFNRRARKPWASVNFVTSHDGFTLADVTAYEQKHNEANREDN
NDGHNENCSRNWGVEGPTDDPAILATRKRVARSLIATLLMALGTPMVLAGDESLRTQNGN
NNAYCQDNEISWIDWERANSPDGQQMTAFVARVIALRKQHPLLRETRFLFGDREVLPGLF
DVGWFDEHGDPLTIEAWQDPEGRAFTLRRAGPGLNGETEVLLMMLNANEETLRFTPPAPH
LEWHVLLDTAEPESAPHRLAVGEVEVAAHSLVMLAAQPVGEADWQAGWKAGAQHGPRLLT
ALPPDPGAHPPSSDTSPTT